1132-61-2 Usage
Description
3-Morpholinopropanesulfonic acid, also known as MOPS, is an N-substituted amino sulfonic acid with a morpholinic ring. It is a Good's buffer substance with a pKa of 7.2 at 20°C. MOPS is capable of buffering within a pH range of 6.5-7.9 and is widely used in biological and biochemical studies due to its inert properties. It does not interact with any metal ions in solutions and has significant metal-buffer stability, especially with copper (Cu), nickel (Ni), manganese (Mn), zinc (Zn), and cobalt (Co) ions.
Uses
Used in Cell Culture:
3-Morpholinopropanesulfonic acid is used as a cell culture additive component in lentiviral particle production. It helps maintain the pH of the mammalian cell culture medium, ensuring optimal growth conditions for the cells.
Used in Microbial Growth Medium:
MOPS is used as a buffering agent in microbial growth medium, providing a stable pH environment for the growth and metabolism of microorganisms.
Used in Nuclei Extraction Buffer:
3-Morpholinopropanesulfonic acid is used as a buffering agent in nuclei extraction buffer, helping to maintain the pH during the process of isolating nuclei from cells.
Used in RPMI Medium:
MOPS is used as a component of Roswell Park Memorial Institute (RPMI) medium for diluting fungal inoculum, ensuring a stable pH for fungal growth.
Used in Capillary-Zone Electrophoresis:
3-Morpholinopropanesulfonic acid is used as a buffer in capillary-zone electrophoresis to test performance, providing a stable pH environment for the separation of molecules.
Used in Algal Sample Processing:
MOPS is used for the dilution of proteins from algal samples, maintaining a stable pH during the process.
Used in Denaturing Gel Electrophoresis of RNA:
3-Morpholinopropanesulfonic acid functions to maintain pH in denaturing gel electrophoresis of RNA, ensuring accurate separation and analysis of RNA molecules.
Used in Lipid Interactions and Membrane Properties:
MOPS can modify lipid interactions and influence the thickness and barrier properties of membranes, making it useful in studies involving membrane structure and function.
Used in Protein Stabilization:
3-Morpholinopropanesulfonic acid interacts with bovine serum albumin and stabilizes the protein, making it a useful component in protein storage and handling.
Used in Polyacrylamide Gel Electrophoresis:
MOPS is frequently used as a buffering agent in biology and biochemistry, including polyacrylamide gel electrophoresis, providing a stable pH environment for the separation of proteins or nucleic acids.
Reference
P. H. Quail, D. Marme, E. Sch?fer, Particle-bound phytochrome from maize and pumpkin, Nature New Biology, 1973, vol. 245, pp. 189-191
Flammability and Explosibility
Notclassified
Check Digit Verification of cas no
The CAS Registry Mumber 1132-61-2 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 1,1,3 and 2 respectively; the second part has 2 digits, 6 and 1 respectively.
Calculate Digit Verification of CAS Registry Number 1132-61:
(6*1)+(5*1)+(4*3)+(3*2)+(2*6)+(1*1)=42
42 % 10 = 2
So 1132-61-2 is a valid CAS Registry Number.
InChI:InChI=1/C7H15NO4S/c9-13(10,11)7-1-2-8-3-5-12-6-4-8/h1-7H2,(H,9,10,11)
1132-61-2Relevant articles and documents
Preparation method of biological buffer agent--moropholino ethanesulfonic acid (MOPS)
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Paragraph 0023-0027; 0030-0033, (2019/07/04)
The invention belongs to the field of organic synthesis and specifically relates to a preparation method of a biological buffer agent--moropholino ethanesulfonic acid (MOPS), comprising the followingstep: morpholine and 1,3-propane sultone (1,3-PS) react to generate moropholino ethanesulfonic acid. The raw materials used in the method are cheaper than raw materials used in the method for synthesizing moropholino ethanesulfonic acid by using 1,3-dibromopropane and morpholine, and the method of the invention is beneficial to large-scale production and application. by using ethanol and the likeas a solvent, the solvent is easy to remove, thus meeting the requirements of users on solvent residue. In the method, a reducing agent is added and nitrogen protection method is adopted for decoloration, thus ensuring chroma of MOPS and making the properties of the product to be better. The preparation process is simple and is convenient to operate and has high practicality.
Aromatic compounds Mannich reaction using economical acidic ionic liquids based on morpholinium salts as dual solvent-catalysts
Yue, Caibo
experimental part, p. 3640 - 3647 (2010/12/25)
Economical acidic ionic liquids containing an alkanesulfonic acid group in a morpholinium cation were found to be effective catalysts for the Mannich reactions of various kinds of aromatic aldehydes, acetophenone and aromatic amines at mild reaction conditions. The satisfactory results were obtained with short reaction time, good yields of the Mannich bases and simplicity in the experimental procedure. These ionic liquids could be recycled and reused for up to five times without considerable decreasing in the catalytic activity. Copyright
Isolation of nucleic acids
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, (2008/06/13)
A method for extracting nucleic acids from a biological material such as blood comprises contacting the mixture with a material at a pH such that the material is positively charged and will bind negatively charged nucleic acids and then eluting the nucleic acids at a pH when the said materials possess a neutral or negative charge to release the nucleic acids. The nucleic acids can be removed under mildly alkaline conditions to the maintain integrity of the nucleic acids and to allow retrieval of the nucleic acids in reagents that are immediately compatible with either storage or analytical testing.
