115035-46-6 Usage
Description
H-GLY-PRO-AMC HBR, also known as Gly-Pro-7-amido-4-methylcoumarin hydrobromide, is a synthetic peptide substrate used in biochemical research. It is composed of two amino acids, glycine and proline, with a 7-amido-4-methylcoumarin group attached. This molecule is characterized by its ability to be cleaved by specific enzymes, making it a valuable tool for studying enzyme activity and function.
Uses
Used in Pharmaceutical Research:
H-GLY-PRO-AMC HBR is used as a substrate for determining the dipeptidyl peptidase IV (DPPIV) activity of various extracts and compounds. This application is particularly relevant in the field of pharmaceutical research, as DPPIV is an enzyme that plays a role in the regulation of immune responses and has been implicated in various diseases, including type 2 diabetes, cancer, and autoimmune disorders.
Used in Enzyme Assays:
In the field of biochemistry, H-GLY-PRO-AMC HBR is used as a substrate in enzyme assays to measure the activity of DPPIV and other related enzymes. The cleavage of the peptide bond by the enzyme releases the fluorescent 7-amido-4-methylcoumarin group, allowing for the quantification of enzyme activity through fluorescence detection.
Used in Drug Development:
H-GLY-PRO-AMC HBR is also utilized in the development of new drugs targeting DPPIV. By using this substrate, researchers can screen for potential inhibitors of the enzyme, which may lead to the development of novel therapeutics for diseases associated with DPPIV dysregulation.
Used in Analytical Chemistry:
In analytical chemistry, H-GLY-PRO-AMC HBR can be employed as a reference compound for the calibration of instruments used in the detection and quantification of peptides and proteins. Its well-defined structure and fluorescence properties make it a suitable standard for validating the performance of analytical techniques.
Used in Quality Control:
Pharmaceutical and biotechnology companies may use H-GLY-PRO-AMC HBR in quality control processes to ensure the purity and potency of DPPIV inhibitors and other related compounds. By measuring the enzyme activity in the presence of these compounds, manufacturers can verify the effectiveness of their products and maintain consistent quality standards.
Check Digit Verification of cas no
The CAS Registry Mumber 115035-46-6 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,1,5,0,3 and 5 respectively; the second part has 2 digits, 4 and 6 respectively.
Calculate Digit Verification of CAS Registry Number 115035-46:
(8*1)+(7*1)+(6*5)+(5*0)+(4*3)+(3*5)+(2*4)+(1*6)=86
86 % 10 = 6
So 115035-46-6 is a valid CAS Registry Number.
InChI:InChI=1/C17H19N3O4.BrH/c1-10-7-16(22)24-14-8-11(4-5-12(10)14)19-17(23)13-3-2-6-20(13)15(21)9-18;/h4-5,7-8,13H,2-3,6,9,18H2,1H3,(H,19,23);1H
