117620-77-6 Usage
Description
3-Cyano-7-ethoxycoumarin is a fluorescent substrate used for the determination of cytochrome P-450 dependent oxidases. It is a compound that exhibits high sensitivity in detecting these enzymes due to its higher turnover rate compared to conventional substrates.
Uses
Used in Enzyme Activity Detection:
3-Cyano-7-ethoxycoumarin is used as a substrate for the determination of cytochrome P-450 dependent oxidases, providing a more sensitive detection method than traditional substrates like ethoxy-resorufin. This increased sensitivity is attributed to its higher turnover rate, making it a valuable tool in research and diagnostic applications.
Used in Cytochrome P (CYP) Inhibition Studies:
3-Cyano-7-ethoxycoumarin has been utilized as a substrate in cytochrome P (CYP) inhibition assays for fluorescence measurement. This application aids in understanding the interactions between CYP enzymes and potential inhibitors, which is crucial for drug development and assessing drug-drug interactions.
Used in Pharmaceutical Research:
As a fluorescent substrate for various cytochrome P450 enzymes, including CYP1A1, CYP1A2, CYP2C9, and CYP2C19, 3-Cyano-7-ethoxycoumarin is used in the pharmaceutical industry for drug metabolism studies. This helps in predicting the metabolic fate of new drug candidates and assessing their potential for drug-drug interactions.
Used in Analytical Chemistry:
In the field of analytical chemistry, 3-Cyano-7-ethoxycoumarin serves as a valuable reagent for the detection and quantification of cytochrome P-450 dependent oxidases. Its high sensitivity and specificity make it an ideal choice for developing new analytical methods and improving existing ones.
Used in Biomedical Research:
3-Cyano-7-ethoxycoumarin is employed in biomedical research to study the role of cytochrome P-450 enzymes in various physiological and pathological processes. This includes investigating their involvement in drug metabolism, detoxification, and the development of certain diseases.
Biochem/physiol Actions
Fluorescent probe useful in microsomal dealkylase studies. Typical drug-drug interactions resulting from enzyme inhibition.
Check Digit Verification of cas no
The CAS Registry Mumber 117620-77-6 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,1,7,6,2 and 0 respectively; the second part has 2 digits, 7 and 7 respectively.
Calculate Digit Verification of CAS Registry Number 117620-77:
(8*1)+(7*1)+(6*7)+(5*6)+(4*2)+(3*0)+(2*7)+(1*7)=116
116 % 10 = 6
So 117620-77-6 is a valid CAS Registry Number.
InChI:InChI=1/C12H9NO3/c14-11(8-4-2-1-3-5-8)9-6-10(12(15)16)13-7-9/h1-7,13H,(H,15,16)
