118362-03-1 Usage
Description
5'-O-(4,4-Dimethoxytrityl)-2'-O-[(tert-butyl)dimethylsilyl]uridine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite is a complex organic compound that is primarily used in the field of molecular biology and biochemistry. It is a modified nucleotide that plays a crucial role in the synthesis of RNA and DNA molecules.
Uses
Used in Molecular Biology:
5'-O-(4,4-Dimethoxytrityl)-2'-O-[(tert-butyl)dimethylsilyl]uridine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite is used as a building block for the synthesis of RNA and DNA molecules. It is particularly useful in the study of efficient enzyme-free copying of all four nucleobases templated by immobilized RNA, which can be crucial for understanding the fundamental processes of genetic information transfer and replication.
Used in Biochemical Research:
In the field of biochemical research, this compound is used as a reagent for the synthesis of modified nucleic acids, which can help researchers investigate the structure, function, and interactions of RNA and DNA molecules. The use of this phosphoramidite allows for the incorporation of specific modifications into the nucleic acid sequence, enabling the study of their effects on molecular recognition, stability, and biological activity.
Used in Drug Development:
5'-O-(4,4-Dimethoxytrityl)-2'-O-[(tert-butyl)dimethylsilyl]uridine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite may also be used in the development of new drugs targeting RNA and DNA molecules. By incorporating this modified nucleotide into the drug design process, researchers can potentially develop novel therapeutic agents that can interact with specific RNA or DNA sequences, leading to the modulation of gene expression or the inhibition of viral replication.
Used in Diagnostics:
In the diagnostics industry, this compound can be employed in the development of new diagnostic tools and assays for the detection and analysis of RNA and DNA molecules. The use of this phosphoramidite in the synthesis of modified nucleic acids can improve the sensitivity and specificity of diagnostic tests, allowing for the accurate identification of genetic mutations, viral infections, or other molecular markers associated with various diseases.
Check Digit Verification of cas no
The CAS Registry Mumber 118362-03-1 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,1,8,3,6 and 2 respectively; the second part has 2 digits, 0 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 118362-03:
(8*1)+(7*1)+(6*8)+(5*3)+(4*6)+(3*2)+(2*0)+(1*3)=111
111 % 10 = 1
So 118362-03-1 is a valid CAS Registry Number.
118362-03-1Relevant articles and documents
Microwave-assisted preparation of nucleoside-phosphoramidites
Meher,Efthymiou,Stoop,Krishnamurthy
supporting information, p. 7463 - 7465 (2014/07/07)
Microwave-assisted phosphitylation of sterically hindered nucleosides is demonstrated to be an efficient method for the preparation of corresponding phosphoramidites (otherwise onerous under standard conditions) and is shown to be general in its applicability. the Partner Organisations 2014.
Methods of producing phosphitylated compounds
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Page 6-7, (2008/06/13)
Provided are methods of producing phosphitylated compounds, including 3′-O-phosphoramidites, comprising the step of reacting a hydroxyl-containing compound with a phosphitylating agent in the presence of a phosphitylation activator selected from the group consisting of: (1) acid-base complexes derived from an amine base of Formula I 1wherein R, R1, and R2 are independently C1-C10 alkyl, C1-C10 cycloalkyl, C1-C10 aryl, C1-C10 aralkyl, C1-C10 heteroalkyl, or C1-C10 heteroaryl; (2) acid-base complexes derived from an amine base of Formula II 2wherein R3, R4, R5, R6, and R7 are independently hydrogen, C1-C10 alkyl, C1-C10 cycloalkyl, C1-C10 aryl, C1-C10 aralkyl, C1-C10 heteroalkyl, or C1-C10 heteroaryl, and at least one of R3, R4, R5 R6, and R7 is not hydrogen.; (3) acid-base complexes derived from a diazabicyclo amine base; (4) zwitterionic amine complexes; and (5) combinations of two or more thereof, to produce a phosphitylated compound.
Methods for synthesizing nucleosides, nucleoside derivatives and non-nucleoside derivatives
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, (2008/06/13)
The present invention provides methods for the chemical synthesis of nucleosides and derivatives thereof, including 2′-amino, 2′-N-phthaloyl, 2′-O-methyl, 2′-O-silyl, 2′-O-triisopropylsilyloxymethyl, 2′-OH nucleosides, C-nucleosides, nucleoside phosphoramidites, C-nucleoside phosphoramidites, and non-nucleoside derivatives.
