119520-58-0 Usage
Description
Arachidonoyl p-Nitroaniline (APNA) is a nitroaniline fatty acid amide used as a probe to measure the activity of fatty acid amide hydrolase (FAAH). FAAH is an enzyme that accepts various amide head groups and hydrolyzes fatty acid amides with fewer carbons and double bonds than arachidonate. APNA serves as a valuable tool for studying FAAH activity due to its ability to release the yellow colorimetric dye p-nitroaniline upon exposure to FAAH, allowing for fast and convenient measurements using a 96-well plate spectrophotoometer.
Uses
Used in Pharmaceutical Research:
APNA is used as a research tool for studying the activity of fatty acid amide hydrolase (FAAH) in the pharmaceutical industry. This is important for understanding the role of FAAH in various biological processes and its potential as a therapeutic target for conditions related to its activity.
Used in Enzyme Activity Assays:
APNA is used as a substrate in enzyme activity assays to measure the activity of FAAH. The release of the yellow colorimetric dye p-nitroaniline upon hydrolysis by FAAH allows for quick and efficient assessment of enzyme activity, which can be crucial in drug discovery and development processes.
Used in Analytical Chemistry:
In the field of analytical chemistry, APNA is used for the determination of FAAH activity levels. The colorimetric assay involving APNA provides a convenient and reliable method for quantifying FAAH activity, which can be useful in various research applications and quality control processes.
Check Digit Verification of cas no
The CAS Registry Mumber 119520-58-0 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,1,9,5,2 and 0 respectively; the second part has 2 digits, 5 and 8 respectively.
Calculate Digit Verification of CAS Registry Number 119520-58:
(8*1)+(7*1)+(6*9)+(5*5)+(4*2)+(3*0)+(2*5)+(1*8)=120
120 % 10 = 0
So 119520-58-0 is a valid CAS Registry Number.
119520-58-0Relevant articles and documents
THE INVENTION OF RADICAL REACTIONS PART XVIII. A CONVENIENT SOLUTION TO THE 1-CARBON PROBLEM (R-CO2H --> R-13CO2H)
Barton, Derek H. R.,Ozbalik, Nubar,Vacher, Bernard
, p. 3501 - 3512 (2007/10/02)
Radicals generated by photolysis (W light) of esters derived from N-hydroxy-2-thiopyridone react with electrophilic isocyanides 2a and (in the presence of trifluoroacetic acid) 2b to give adducts of type 3.Convenient reaction procedures have been worked out to hydrolyse the adducts to amides of type 5, from which the original acid can be regenerated under mild conditions.The three important acids oleic, linoleic and arachidonic have all given smooth reactions.In suitable examples, quantitative evolution of carbon dioxide and incorporation of 13C without dilution have been demonstrated.This reaction sequence will be useful for the labelling in the carboxyl group of prostaglandins, leukotrienes, and the side chain carboxyls of peptides.
