119616-38-5 Usage
Description
BIOTIN-NHS, WATER-SOLUBLE is a biotinylation reagent that contains a sulfo-NHS moiety and a biotin group. It is designed for the conjugation of biotin to antibodies or proteins, enabling their detection or immobilization in various applications. The sulfo-NHS group allows for the control and modification of carbodiimide crosslinking reactions, while the terminal biotinylation can react with amine molecules in the presence of activators such as EDC or HATU.
Uses
Used in Apoptosis Probes:
BIOTIN-NHS, WATER-SOLUBLE is used as an apoptosis probe for detecting and analyzing apoptotic cells. It can be conjugated to antibodies or proteins that specifically bind to markers of apoptosis, allowing for the identification and quantification of dying cells.
Used in Cell Permeable Fluorescent Ca2+-Sensitive Dye for Cytosolic Ca2+ Assays:
BIOTIN-NHS, WATER-SOLUBLE is used as a cell permeable fluorescent Ca2+-sensitive dye for measuring cytosolic Ca2+ levels in cells. It can be conjugated to a fluorescent dye that is sensitive to changes in Ca2+ concentration, enabling the monitoring of intracellular Ca2+ dynamics in response to various stimuli.
Used in Biotinylation of Antibodies or Proteins for Protein Detection or Immobilization Studies:
BIOTIN-NHS, WATER-SOLUBLE is used as a biotinylation reagent for antibodies or proteins that can then be used for protein detection or immobilization studies. It is particularly effective when targeting cell surface proteins, as it allows for the specific labeling and detection of these proteins using streptavidin or immobilized streptavidin.
BIOTIN-NHS, WATER-SOLUBLE is used in biotin:avidin second antibody signal systems, where it is conjugated to a secondary antibody. Once the secondary antibody binds to a target primary antibody in an immunochemical assay, the signal is developed by allowing avidin, which has been conjugated to an enzyme or signal molecule, to bind to the antibody-associated biotin molecule. This enhances the sensitivity and specificity of the assay.
Used in Research and Diagnostic Applications:
BIOTIN-NHS, WATER-SOLUBLE is used in various research and diagnostic applications, including enzyme-linked immunosorbent assays (ELISA), Western blotting, immunohistochemistry, and flow cytometry. Its water-soluble property makes it suitable for use in aqueous solutions and buffers, facilitating its incorporation into various experimental protocols.
Biological Activity
sulfo-nhs-biotin (n-hydroxysulfosuccinimidobiotin) is an amine-reactive biotinylation reagent. the most common amine-reactive biotinylation reagents can be divied into two basic types, n-hydroxysuccinimide (nhs) esters and carboxylates. generally, nhs esters biotinylation reagent contains a reactive nhs ring structure that reacts with an amine on the carbonyl group of a protein or other molecules through nucleophilic attack subsequently forming a stable amide linkage and releasing the nhs group. due to the insolubility of nhs-biotin in aqueous environments, sulfo-nhs-biotin, a water-soluble analog of nhs-biotin, has been developed by attaching a negatively charged sulfonate group on the nhs ring structure, which can be added directly to aqueous reactions without the need for organic solvent dissolution.bioconjugate techniques , 2nd ed. by greg t.hermanson (pierce biotechnology, thermo fisher scientific, rockford, il). academic press (an imprint of elsevier): london, amsterdam, burlington, san diego . 2008. isbn 978-0-12-370501-3.
Check Digit Verification of cas no
The CAS Registry Mumber 119616-38-5 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,1,9,6,1 and 6 respectively; the second part has 2 digits, 3 and 8 respectively.
Calculate Digit Verification of CAS Registry Number 119616-38:
(8*1)+(7*1)+(6*9)+(5*6)+(4*1)+(3*6)+(2*3)+(1*8)=135
135 % 10 = 5
So 119616-38-5 is a valid CAS Registry Number.
InChI:InChI=1/C14H19N3O7S2/c18-10(17-11(19)5-9(13(17)20)26(22,23)24)4-2-1-3-8-12-7(6-25-8)15-14(21)16-12/h7-9,12H,1-6H2,(H2,15,16,21)(H,22,23,24)/t7-,8-,9?,12+/m0/s1
