121714-22-5

Basic information

• Product Name: Fluo 3-AM
• Synonyms: 1-[2-AMINO-5-(2,7-DICHLORO-6-ACETOMETHOXY-3-OXO-3H-XANTHEN-9-YL)PHENOXY]-2-(2'-AMINO-5'-METHYLPHENOXY)ETHANE-N,N,N',N'TETRAACETIC ACID PENTAACETOXY-METHYL ESTER;1-[2-AMINO-5-(2,7-DICHLORO-6-HYDROXY-3-OXO-3H-XANTHEN-9-YL)]-2-(2'-AMINO-5' METHYLPHENOXY)ETHANE-N,N,N',N'-TETRAACETIC ACID PENTAACETOXYMETHYL ESTER;4-(6-ACETOXYMETHOXY-2,7-DICHLORO-3-OXO-9-XANTHENYL)-4'-METHYL-2,2'(ETHYLENEDIOXY)DIANILINE-N,N,N',N'-TETRAACETIC ACID TETRAKIS(ACETOXYMETHYL) ESTER;FLUO-3, AM;4-(6-Acetoxymethoxy-2,7-dichloro-3-oxo-9-xanthenyl)-4'-methyl-2,2′(ethylenedioxy)dianiline-N,N,N′,N'-tetraacetic acid tetrakis(acetoxymethyl) ester;FLUO 3/AM (1SET = 10x100UG);FLUO 3-AM, FOR FLUORESCENCE;1-[2-Amino-5-(2,7-dichloro-6-hydroxy-3-oxo-9-xanthenyl)phenoxy]-2-(2-amino-5-methylphenoxy)ethane-N,N,N',N'-tetraaceticacid,pentaacetoxymethylester
• CAS NO: 121714-22-5
• Molecular Formula: C48H46Cl2N2O21
• Molecular Weight: 1057.79
• Product Categories: Xanthene
• Mol File: 121714-22-5.mol
• Article Data: 0

Chemical Properties

• Melting Point: >250℃
• Boiling Point: 1090.9±65.0 °C(Predicted)
• Flash Point: 87℃
• Appearance: Dark red/crystals
• Density: 1.49±0.1 g/cm3(Predicted)
• Storage Temp.: −20°C
• Solubility: DMSO: soluble
• PKA: 1.92±0.50(Predicted)
• CAS DataBase Reference: Fluo 3-AM(CAS DataBase Reference)
• NIST Chemistry Reference: Fluo 3-AM(121714-22-5)
• EPA Substance Registry System: Fluo 3-AM(121714-22-5)

Safety Data

• Safety Statements: 22-24/25
• RIDADR: NA 1993 / PGIII
• WGK Germany: 3
• F: 1-8-10
• Hazardous Substances Data: 121714-22-5(Hazardous Substances Data)

Usage

Description

Fluo 3-AM is a fluorescent calcium indicator that is widely used in biological research. It is a cell-permeable acetoxymethyl (AM) ester form of the Fluo 3 fluorescent dye, which can be easily loaded into cells and then hydrolyzed by intracellular esterases to yield the membrane-permeable Fluo 3 dye. Fluo 3-AM is a red solid that exhibits strong fluorescence upon binding to calcium ions, making it an ideal tool for monitoring intracellular calcium levels and studying various cellular processes.

Uses

Used in Biological Research:
Fluo 3-AM is used as an apoptosis probe for detecting and quantifying the process of programmed cell death. It helps researchers to understand the mechanisms underlying apoptosis and its role in various diseases and conditions.
Used in Calcium Imaging:
Fluo 3-AM is used as a visible wavelength calcium probe for indicating changes in intracellular Ca2+ levels. It allows researchers to monitor calcium dynamics in real-time, providing valuable insights into various cellular processes such as neurotransmission, muscle contraction, and cell signaling.
Used in Flow Cytometry:
Fluo 3-AM is used with visible-light excitation sources in flow cytometry for the analysis of cellular calcium levels. This technique enables the simultaneous measurement of multiple parameters, providing a comprehensive understanding of cell populations and their responses to various stimuli.
Used in Confocal Laser-Scanning Microscopy:
Fluo 3-AM is used in confocal laser-scanning microscopy for high-resolution imaging of intracellular calcium levels. This technique allows for the visualization of calcium dynamics within specific cellular compartments, providing detailed information on the spatial and temporal aspects of calcium signaling.