121788-83-8

Basic information

• Product Name: (S)-4-Azidocarbonyl-2-{4-[(2,4-diamino-pteridin-6-ylmethyl)-methyl-amino]-benzoylamino}-butyric acid
• Synonyms: (S)-4-Azidocarbonyl-2-{4-[(2,4-diamino-pteridin-6-ylmethyl)-methyl-amino]-benzoylamino}-butyric acid
• CAS NO: 121788-83-8
• Molecular Weight: 479.458
• Mol File: 121788-83-8.mol
• Article Data: 1

Chemical Properties

• CAS DataBase Reference: (S)-4-Azidocarbonyl-2-{4-[(2,4-diamino-pteridin-6-ylmethyl)-methyl-amino]-benzoylamino}-butyric acid(CAS DataBase Reference)
• NIST Chemistry Reference: (S)-4-Azidocarbonyl-2-{4-[(2,4-diamino-pteridin-6-ylmethyl)-methyl-amino]-benzoylamino}-butyric acid(121788-83-8)
• EPA Substance Registry System: (S)-4-Azidocarbonyl-2-{4-[(2,4-diamino-pteridin-6-ylmethyl)-methyl-amino]-benzoylamino}-butyric acid(121788-83-8)

Safety Data

• Hazardous Substances Data: 121788-83-8(Hazardous Substances Data)

Upstream product

• 59-05-2 N-[4-[[(2,4-diamino-6-pteridinyl)methyl]methylamino]benzoyl]-L-glutamic acid 
• 79648-88-7 α-tert-butyl γ-methyl N'-<4--N-methylamino>benzoyl>-L-glutamate 
• 67022-39-3 γ-methyl N'-<4--N-methylamino>benzoyl>-L-glutamate 
• 10541-83-0 N-methyl-p-aminobenzoic acid 
• 79640-75-8 N'-<4--N-methylamino>benzoyl>-L-glutamic acid γ-hydrazide 

Relevant articles and documents

Synthesis of methotrexate-antibody conjugates by regiospecific coupling and assessment of drug and antitumor activities

In order to increase the retention of drug activity, regiospecific coupling has been used to synthesize conjugates of methotrexate (MTX, 1) with normal rabbit IgG (NRG) and a mouse anti-human renal cancer monoclonal IgG (Dal K-20). MTX γ-methyl ester (4) was produced either by selective esterification of MTX or by coupling of 4-amino-4-deoxy-N10-methylpteroic acid (2) with suitable glutamic acid derivatives. The MTX γ-methyl ester (4) was then converted to the corresponding hydrazide 6. An amide-linked conjugate was formed when the MTX γ-hydrazide (6) was converted to reactive acylating species 7 by using tert-butyl nitrite or trifluoroacetaldehyde, which were reacted with nucleophilic centers, presumably ε-amino groups, in native IgG. A hydrazone-linked conjugate was formed when MTX γ-hydrazide (6) was reacted directly with IgG that had first been oxidized with periodate to form polyaldehyde IgG. The regiospecifically synthesized conjugates were somewhat more effective inhibitors in vitro of dihydrofolate reductase and of colony formation by human renal cancer (Caki-1) cells than were control nonregiospecific conjugates.