125455-61-0 Usage
Description
GLY-ARG P-NITROANILIDE DIHYDROCHLORIDE, also known as H-Gly-Arg-pNA, is a colorimetric substrate specifically designed for the detection and quantification of thrombin activity. It consists of a Gly-Arg (GR) peptide sequence that is selectively bound and cleaved by thrombin, resulting in the release of p-nitroanilide (pNA). The released pNA can be easily quantified by colorimetric detection at 405 nm, providing a reliable and sensitive method for measuring thrombin activity.
Uses
Used in Research and Diagnostic Applications:
GLY-ARG P-NITROANILIDE DIHYDROCHLORIDE is used as a colorimetric substrate for thrombin activity assays. It allows researchers and diagnosticians to accurately measure thrombin activity levels in various samples, such as blood, plasma, or cell lysates. This information is crucial for understanding the role of thrombin in various physiological and pathological processes, including blood clotting, inflammation, and wound healing.
Used in Pharmaceutical Industry:
In the pharmaceutical industry, GLY-ARG P-NITROANILIDE DIHYDROCHLORIDE is used as a tool for the development and testing of new antithrombotic drugs. By providing a sensitive and specific method for measuring thrombin activity, this substrate enables researchers to evaluate the efficacy and safety of potential thrombin inhibitors, contributing to the development of novel therapeutic agents for the treatment of thrombotic disorders and related conditions.
Used in Quality Control:
GLY-ARG P-NITROANILIDE DIHYDROCHLORIDE is also employed in the quality control of thrombin reagents and diagnostic kits. It serves as a standard substrate for assessing the performance and reliability of thrombin assays, ensuring that these diagnostic tools provide accurate and consistent results when used in clinical settings.
Check Digit Verification of cas no
The CAS Registry Mumber 125455-61-0 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,2,5,4,5 and 5 respectively; the second part has 2 digits, 6 and 1 respectively.
Calculate Digit Verification of CAS Registry Number 125455-61:
(8*1)+(7*2)+(6*5)+(5*4)+(4*5)+(3*5)+(2*6)+(1*1)=120
120 % 10 = 0
So 125455-61-0 is a valid CAS Registry Number.
125455-61-0Relevant articles and documents
A convenient synthesis of amino acid p-nitroanilides; synthons in the synthesis of protease substrates
Rijkers, Dirk T. S.,Adams, Hans P. H. M.,Hemker, H. Coenraad,Tesser, Godefridus I.
, p. 11235 - 11250 (2007/10/02)
A method is described for the synthesis of N(α)-protected bi- and trifunctional amino acid p-nitroanilides. The reaction uses phosphorus oxychloride as the condensing agent. The synthesis is simple, rapid, free of racemization and affords yields between 70-90%. The synthesis can be performed not only with amino acid derivatives of the urethane type including acid-labile (Z, Boc) and base-labile (Fmoc, Msc) N(α)-protective functions or allyl-derived protections, but also with N(α)-trityl amino acids, albeit in lower yield. The reaction runs in pyridine and its mechanism implies carboxyl activation by formation of a mixed anhydride with phosphorodichloridic acid (HOPOCl2).
