1262883-54-4Relevant articles and documents
Hydrazine visualization fluorescent probe molecule and preparation method thereof
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Paragraph 0031-0037, (2021/09/01)
The invention provides a hydrazine visualization fluorescent probe molecule and a preparation method thereof. The preparation method comprises the steps of mixing compounds of 4-dimethylaminopyridine, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, 3-R-benzoic acid and fluorescein, conducting reaction completely under the room temperature condition, then adding a dichloromethane solvent to obtain a crude product; and purifying the crude product by using a forward silica gel chromatographic column to obtain a white solid fluorescent probe molecule 3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl bis(3-R benzoate) for detecting hydrazine. When hydrazine reacts with a fluorescent probe molecule ester bond, a recognition part is removed, a fluorescence emission center is separated, fluorescein with strong column green fluorescence emission is generated, and qualitative detection of hydrazine is realized through fluorescence color signal change. The method is simple in preparation process and low in cost, has excellent detection selectivity and sensitivity, can be used for rapidly and qualitatively detecting hydrazine, and has a wide application prospect.