127062-22-0 Usage
Description
SULPHOSUCCINIMIDYL-6-(BIOTINAMIDO)HEXANOATE, also known as Biotinamidohexanoic acid 3-sulfo-N-hydroxysuccinimide ester sodium salt, is a water-soluble biotinylation reagent that incorporates an aminocaproyl spacer. This feature helps reduce steric hindrance in binding avidin to some biotinylated compounds. It is commonly used in various applications, including immunoprecipitation of signaling proteins, analysis of hyaluronan synthesis, and ELISA. The reagent has demonstrated specificity for human dermal fibroblasts and is available in a powder form.
Uses
Used in Biotechnology Industry:
SULPHOSUCCINIMIDYL-6-(BIOTINAMIDO)HEXANOATE is used as a biotinylation reagent for the biotinylation of cell surface proteins. This application is particularly useful in reducing steric hindrance in binding avidin to biotinylated compounds, which can enhance the efficiency of various biotechnological processes.
Used in Immunoprecipitation:
In the field of molecular biology, SULPHOSUCCINIMIDYL-6-(BIOTINAMIDO)HEXANOATE is used as a biotinylation reagent for the immunoprecipitation of signaling proteins. This process allows for the isolation and analysis of specific proteins involved in cellular signaling pathways, contributing to a better understanding of cellular processes and potential therapeutic targets.
Used in Hyaluronan Synthesis Analysis:
SULPHOSUCCINIMIDYL-6-(BIOTINAMIDO)HEXANOATE is employed as a biotinylation reagent in the analysis of hyaluronan synthesis. Hyaluronan is an essential component of the extracellular matrix and has various roles in cellular processes, including cell adhesion, migration, and proliferation. The reagent aids in the study of hyaluronan synthesis and its regulation, which can have implications in the development of treatments for various diseases.
Used in Enzyme-Linked Immunosorbent Assay (ELISA):
In the field of immunology, SULPHOSUCCINIMIDYL-6-(BIOTINAMIDO)HEXANOATE is used as a biotinylation reagent in ELISA. This technique is widely used for the detection and quantification of specific antibodies or antigens in a sample. The reagent's biotinylation capability enhances the sensitivity and specificity of the assay, making it a valuable tool in diagnostic applications.
Used in Cell Surface Labeling:
SULPHOSUCCINIMIDYL-6-(BIOTINAMIDO)HEXANOATE is used as a cell surface labeling reagent, allowing for the identification and tracking of specific cell surface proteins. This application is particularly useful in cell biology research, where understanding the role of cell surface proteins in various cellular processes is crucial for the development of new therapeutic strategies.
Biological Activity
sulfo-nhs-lc-biotin (sulfosuccinimidyl 6-(biotinamido) hexanoate) is an amine-reactive biotinylation agent. as a water-soluble analog of nhs-lc-biotin, sulfo-nhs-lc-biotin contains a negatively charged sulfonate group on the nhs ring structure, which creates sufficient polarity within the molecule and allows it to be directly added into aqueous reactions without prior dissolution of organic solvents. sulfo-nhs-lc-biotin covalently binds to amine groups in proteins and other molecules through its nhs ester forming an amide linkage and releasing n-hydroxysuccinimide. sulfo-nhs-lc-biotin has a greater length (22.4 a) between a covalently modified molecule and the bicyclic biotin rings than nhs-biotin (13.5 a), which provides the probes a greater opportunity to reach the binding sites deeply buried inside the surface plane of proteins.bioconjugate techniques , 2nd ed. by greg t.hermanson (pierce biotechnology, thermo fisher scientific, rockford, il). academic press (an imprint of elsevier): london, amsterdam, burlington, san diego . 2008. isbn 978-0-12-370501-3.
Check Digit Verification of cas no
The CAS Registry Mumber 127062-22-0 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,2,7,0,6 and 2 respectively; the second part has 2 digits, 2 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 127062-22:
(8*1)+(7*2)+(6*7)+(5*0)+(4*6)+(3*2)+(2*2)+(1*2)=100
100 % 10 = 0
So 127062-22-0 is a valid CAS Registry Number.
InChI:InChI=1/C20H30N4O9S2/c25-15(7-4-3-6-13-18-12(11-34-13)22-20(29)23-18)21-9-5-1-2-8-17(27)33-24-16(26)10-14(19(24)28)35(30,31)32/h12-14,18H,1-11H2,(H,21,25)(H2,22,23,29)(H,30,31,32)/t12-,13-,14?,18+/m0/s1
