1309375-16-3Relevant articles and documents
A direct, efficient method for the preparation of siRNAs containing ribo-like north bicyclo[3.1.0]hexane pseudosugars
Terrazas, Montserrat,Avino, Anna,Siddiqui, Maqbool A.,Marquez, Victor E.,Eritja, Ramon
, p. 2888 - 2891 (2011/08/02)
An efficient method for the preparation of siRNAs modified with ribo-like North bicyclo[3.1.0]hexane pseudosugars is described. The combined use of 2-O-(2-cyanoethoxymethyl) (CEM) and 2-O-TBDMS protection was successfully employed for RNA synthesis with the added advantage that both groups were efficiently removed in a single step. The resulting North ribo-methanocarba- modified siRNAs are compatible with the intracellular RNAi machinery and can mediate specific degradation of target mRNA.