1334952-43-0Relevant articles and documents
A combination of in vivo selection and cell sorting for the identification of enantioselective biocatalysts
Fernandez-Alvaro, Elena,Snajdrova, Radka,Jochens, Helge,Davids, Timo,Boettcher, Dominique,Bornscheuer, Uwe T.
supporting information; experimental part, p. 8584 - 8587 (2011/11/07)
The "carrot and stick" principle could be combined with cell sorting to enable the selection of enantioselective esterase variants from a mutant library. Hence, the enormous diversity generated in directed evolution experiments is now easily accessible by this high-throughput system. In line with the principle, the hydrolysis of 1 glycerin supports cell growth, whereas the hydrolysis of 2 leads to cell death. Copyright
