133628-73-6

Basic information

• Product Name: H-ASP(AMC)-OH
• Synonyms: H-ASP(AMC)-OH;ASPARTIC ACID(AMC)-OH;L-Aspartic acid 4-(4-methyl-7-coumarinylamide);L-ASPARTIC ACID BETA-(7-AMIDO-4-METHYLCOUMARIN);L-ASPARTIC ACID BETA-(7-AMIDO-4-METHYLCO;L-ASPARTIC ACID B-(7-AMIDO4-METHYLCOUMAR IN);L-ASPARTIC ACID ALPHA-(7-AMIDO-4-METHYLCOUMARIN) 1-HYDRATE;l-aspartic acid β-(4-methyl-7-coumarinylamide)
• CAS NO: 133628-73-6
• Molecular Formula: C14H14N2O5
• Molecular Weight: 290.27
• Mol File: 133628-73-6.mol
• Article Data: 0

Chemical Properties

• Boiling Point: 610°Cat760mmHg
• Flash Point: 322.7°C
• Appearance: /
• Density: 1.452g/cm³
• Vapor Pressure: 9.8E-16mmHg at 25°C
• Refractive Index: 1.651
• Storage Temp.: 2-8°C
• Solubility: 1 M HCl: 10 mg/mL, clear, faintly yellow
• PKA: 2.32±0.10(Predicted)
• CAS DataBase Reference: H-ASP(AMC)-OH(CAS DataBase Reference)
• NIST Chemistry Reference: H-ASP(AMC)-OH(133628-73-6)
• EPA Substance Registry System: H-ASP(AMC)-OH(133628-73-6)

Safety Data

• Safety Statements: 22-24/25
• WGK Germany: 3
• Hazardous Substances Data: 133628-73-6(Hazardous Substances Data)

Usage

Description

H-ASP(AMC)-OH, also known as L-Aspartic Acid β-(7-Amido-4-methylcoumarin), is a sensitive fluorogenic substrate commonly utilized in various biochemical and medical applications. It is a white crystalline powder that exhibits unique chemical properties, making it a valuable compound in the field of biochemistry and molecular biology.

Uses

Used in Diagnostic Applications:
H-ASP(AMC)-OH is used as a diagnostic tool for aspartylglycosaaminuria, a rare genetic disorder characterized by the deficiency of the enzyme aspartylglucosaminidase. The compound serves as a substrate in lysosomal glycoasparaginase assays, allowing for the detection and monitoring of the disease's progression.
Used in Pharmaceutical Research:
In the pharmaceutical industry, H-ASP(AMC)-OH is employed as a research tool for the development of new drugs and therapies targeting lysosomal storage disorders and other related conditions. Its unique properties make it an ideal candidate for studying enzyme activity and understanding the underlying mechanisms of these diseases.
Used in Biochemical Assays:
H-ASP(AMC)-OH is used as a sensitive fluorogenic substrate in various biochemical assays, enabling researchers to study enzyme kinetics, monitor enzyme activity, and evaluate the effectiveness of potential therapeutic agents. Its ability to produce a fluorescent signal upon enzymatic cleavage makes it a valuable tool for high-throughput screening and other research applications.
Used in Quality Control:
In the biotechnology and pharmaceutical industries, H-ASP(AMC)-OH is utilized for quality control purposes, ensuring the purity and potency of enzymes and other bioactive compounds. Its sensitivity and specificity make it an ideal choice for assessing the performance of these compounds in a variety of settings.

InChI:InChI=1/C14H14N2O5/c1-7-4-13(18)21-11-5-8(2-3-9(7)11)16-12(17)6-10(15)14(19)20/h2-5,10H,6,15H2,1H3,(H,16,17)(H,19,20)/t10-/m0/s1