13408-09-8 Usage
Description
BETA-GLYCEROL PHOSPHATE DISODIUM SALT, also known as Sodium β-glycerophosphate pentahydrate, is a phosphate group donor that plays a crucial role in matrix mineralization studies. It is a solid substance that acts as a protein phosphatase inhibitor and promotes bone matrix mineralization when delivered to osteoblasts by providing a source of phosphate ions. Additionally, it accelerates calcification of cultured vascular smooth muscle cells through an alkaline phosphatase-related mechanism.
Uses
Used in Tissue Engineering and Cell Growth:
BETA-GLYCEROL PHOSPHATE DISODIUM SALT is used as a component in the development of hydrogels and scaffolds for tissue engineering and cell growth. It serves as a local source of inorganic phosphate ions, promoting bone matrix mineralization and enhancing the overall effectiveness of these engineered constructs.
Used in Isolation Mediums:
In the field of cell culture, BETA-GLYCEROL PHOSPHATE DISODIUM SALT is used as an additive in isolation mediums. It provides phosphate ions necessary for the growth and maintenance of various cell types, particularly osteoblasts, which are crucial for bone formation and repair.
Used in Matrix Mineralization Studies:
BETA-GLYCEROL PHOSPHATE DISODIUM SALT is used as a phosphate group donor in matrix mineralization studies. It modulates bone cell metabolic activity and promotes mineralization in vitro, which is essential for understanding the processes involved in bone formation and the development of potential therapeutic agents for bone-related disorders.
Used as a Phosphatase Inhibitor:
In biochemical research, BETA-GLYCEROL PHOSPHATE DISODIUM SALT is utilized as a protein phosphatase inhibitor. This property allows researchers to study the effects of phosphatase inhibition on various cellular processes and signaling pathways, contributing to the advancement of knowledge in cell biology and the development of novel therapeutic strategies.
Biochem/physiol Actions
Beta-Glycerophosphate is a classical serine-threonine phosphatase inhibitor used in kinase reaction buffers. BGP is often used in combination with other phosphatase/protease inhibitors for broad spectrum inhibition. It functions as an organic phosphate donor and has been used in culture media for mesenchymal stem cell differentiation to osteoblast-type cells. BGP is also used to buffer M17 media for Lactococcus culture in recombinant protein expression
Purification Methods
Crystallise it from water. [Attwood et al. Biochem J 253 389 1988, Beilstein 1 IV 2766.]
references
[1]. chung ch, golub ee, forbes e, et al. mechanism of action of beta-glycerophosphate on bone cell mineralization. calcif tissue int. 1992 oct;51(4):305-11.[2]. fujimoto h, mabuchi i. isolation of cleavage furrows from eggs of regular sea urchins and identification of furrow-specific proteins. j biochem. 1997 sep;122(3):518-24.[3]. lecanda f, avioli lv, cheng sl. regulation of bone matrix protein expression and induction of differentiation of human osteoblasts and human bone marrow stromal cells by bone morphogenetic protein-2. j cell biochem. 1997 dec 1;67(3):386-96.[4]. shioi a, nishizawa y, jono s, et al. beta-glycerophosphate accelerates calcification in cultured bovine vascular smooth muscle cells. arterioscler thromb vasc biol. 1995 nov;15(11):2003-9.
Check Digit Verification of cas no
The CAS Registry Mumber 13408-09-8 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 1,3,4,0 and 8 respectively; the second part has 2 digits, 0 and 9 respectively.
Calculate Digit Verification of CAS Registry Number 13408-09:
(7*1)+(6*3)+(5*4)+(4*0)+(3*8)+(2*0)+(1*9)=78
78 % 10 = 8
So 13408-09-8 is a valid CAS Registry Number.
