135313-63-2 Usage
Description
4-Chloro-3-indolyl beta-D-galactopyranoside, commonly known as X-Gal, is a synthetic compound that serves as a substrate for the enzyme beta-galactosidase. It is a white crystalline powder that is soluble in water and dimethylformamide. X-Gal is widely used in molecular biology and microbiology as a colorimetric indicator due to its ability to produce a blue precipitate upon enzymatic hydrolysis.
Uses
Used in Molecular Biology:
4-Chloro-3-indolyl beta-D-galactopyranoside is used as a colorimetric substrate for the detection of beta-galactosidase activity. When beta-galactosidase acts on X-Gal, it cleaves the molecule, resulting in the formation of a blue-colored product. This property makes X-Gal a valuable tool for identifying and selecting genetically modified cells or organisms that express the beta-galactosidase gene.
Used in Microbiology:
In microbiology, 4-Chloro-3-indolyl beta-D-galactopyranoside is used as a selective agent and indicator for the detection of coliforms, particularly Escherichia coli. When X-Gal is incorporated into a culture medium, it allows for the identification of coliforms based on their ability to ferment lactose and produce beta-galactosidase, which in turn hydrolyzes X-Gal and forms the blue-colored product. This characteristic is utilized in various diagnostic and quality control applications in the food and water industries.
Used in Patented Indicator Platforms:
4-Chloro-3-indolyl beta-D-galactopyranoside is also used as a component in patented indicator platforms, which are designed to detect specific biological activities or the presence of certain microorganisms. These platforms often involve the use of X-Gal in combination with other substrates or reagents to provide a more sensitive and specific detection method. The blue-colored product formed upon enzymatic hydrolysis of X-Gal serves as a visual signal, indicating the presence or activity of the target analyte.
Check Digit Verification of cas no
The CAS Registry Mumber 135313-63-2 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,3,5,3,1 and 3 respectively; the second part has 2 digits, 6 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 135313-63:
(8*1)+(7*3)+(6*5)+(5*3)+(4*1)+(3*3)+(2*6)+(1*3)=102
102 % 10 = 2
So 135313-63-2 is a valid CAS Registry Number.
InChI:InChI=1/C14H16ClNO6/c15-6-2-1-3-7-10(6)8(4-16-7)21-14-13(20)12(19)11(18)9(5-17)22-14/h1-4,9,11-14,16-20H,5H2
