1392497-95-8Relevant articles and documents
Reversible Control of Protein Localization in Living Cells Using a Photocaged-Photocleavable Chemical Dimerizer
Aonbangkhen, Chanat,Zhang, Huaiying,Wu, Daniel Z.,Lampson, Michael A.,Chenoweth, David M.
, p. 11926 - 11930 (2018)
Many dynamic biological processes are regulated by protein-protein interactions and protein localization. Experimental techniques to probe such processes with temporal and spatial precision include photoactivatable proteins and chemically induced dimerization (CID) of proteins. CID has been used to study several cellular events, especially cell signaling networks, which are often reversible. However, chemical dimerizers that can be both rapidly activated and deactivated with high spatiotemporal resolution are currently limited. Herein, we present a novel chemical inducer of protein dimerization that can be rapidly turned on and off using single pulses of light at two orthogonal wavelengths. We demonstrate the utility of this molecule by controlling peroxisome transport and mitotic checkpoint signaling in living cells. Our system highlights and enhances the spatiotemporal control offered by CID. This tool addresses biological questions on subcellular levels by controlling protein-protein interactions.
Photoactivatable trimethoprim-based probes for spatiotemporal control of biological processes
Wu, Daniel Z.,Lampson, Michael A.,Chenoweth, David M.
, p. 273 - 294 (2020)
Optogenetic tools allow regulation of cellular processes with light, which can be delivered with spatiotemporal resolution. By combining the chemical versatility of photoremovable protecting groups with the biological specificity of self-labeling tags, we
