144110-43-0 Usage
Description
5-Bromo-6-chloro-3-indolyl-D-glucuronide cyclohexylammonium salt, commonly known as X-GlcU, is a synthetic chromogenic substrate used in the detection and quantification of β-glucuronidase enzyme activity. It is a white to off-white crystalline powder that, upon enzymatic action, forms a magenta-red precipitate, making it an ideal indicator for various applications.
Uses
Used in Research and Diagnostic Applications:
5-Bromo-6-chloro-3-indolyl-D-glucuronide cyclohexylammonium salt is used as a chromogenic substrate for β-glucuronidase to detect and quantify the enzyme's activity. The formation of a magenta-red precipitate upon enzymatic action allows for the visualization and measurement of β-glucuronidase levels in samples, making it a valuable tool in research and diagnostic applications.
Used in Pharmaceutical Industry:
In the pharmaceutical industry, 5-Bromo-6-chloro-3-indolyl-D-glucuronide cyclohexylammonium salt is used as a quality control agent for the assessment of β-glucuronidase inhibitors. These inhibitors are essential in the development of drugs targeting various diseases, including cancer and inflammatory conditions, where β-glucuronidase plays a role in disease progression.
Used in Environmental Monitoring:
5-Bromo-6-chloro-3-indolyl-D-glucuronide cyclohexylammonium salt is also used in environmental monitoring to detect the presence of β-glucuronidase-producing microorganisms in soil, water, and other ecosystems. This information can be crucial for understanding the ecological balance and assessing the impact of human activities on the environment.
Used in Food Industry:
In the food industry, 5-Bromo-6-chloro-3-indolyl-D-glucuronide cyclohexylammonium salt can be employed as a detection agent for β-glucuronidase activity in the fermentation process. This enzyme plays a role in the breakdown of glucuronic acid, which is essential for the production of certain fermented products. Monitoring β-glucuronidase activity can help ensure the quality and consistency of these products.
Used in Biotechnology:
5-Bromo-6-chloro-3-indolyl-D-glucuronide cyclohexylammonium salt is used in biotechnology for the development of biosensors and high-throughput screening assays. The enzyme's activity can be easily monitored due to the formation of the magenta-red precipitate, making it a convenient and reliable indicator for various applications in biotechnology research and development.
Check Digit Verification of cas no
The CAS Registry Mumber 144110-43-0 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,4,4,1,1 and 0 respectively; the second part has 2 digits, 4 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 144110-43:
(8*1)+(7*4)+(6*4)+(5*1)+(4*1)+(3*0)+(2*4)+(1*3)=80
80 % 10 = 0
So 144110-43-0 is a valid CAS Registry Number.
InChI:InChI=1/C14H13BrClNO7/c15-5-1-4-7(2-6(5)16)17-3-8(4)23-14-11(20)9(18)10(19)12(24-14)13(21)22/h1-3,9-12,14,17-20H,(H,21,22)/p-1/t9-,10-,11+,12-,14+/m0/s1
