146-56-5 Usage
Description
FLUPHENAZINE HYDROCHLORIDE, a member of the piperazine subgroup, is a potent antipsychotic phenothiazine with a trifluoromethyl group at the 2-position of the phenothiazine system. It is known as 4-[3-[2-(trifluoromethyl)phenazin-10-yl]propyl]-1-piperazineethanol dihydrochloride and 10[3-[4-(2-hydroxyethyl)piperazinyl]propyl]-2-trifluoromethylphenothiazine dihydrochloride (Permitil, Prolixin). FLUPHENAZINE HYDROCHLORIDE is also available in two lipid-soluble esters for depot intramuscular injection, the enanthate (heptanoic acid ester) and the decanoate ester. It is an off-white solid and is used for various applications in the medical field.
Uses
Used in Psychiatry:
FLUPHENAZINE HYDROCHLORIDE is used as an antipsychotic agent for the treatment of various psychotic disorders, such as schizophrenia and bipolar disorder. It helps in managing symptoms like hallucinations, delusions, and disorganized thinking.
Used in Hormone Regulation:
FLUPHENAZINE HYDROCHLORIDE is used as an antiandrogen and a nuclear hormone receptor antagonist, playing a role in regulating hormone levels and reducing the effects of androgens in the body.
Used in Oncology:
FLUPHENAZINE HYDROCHLORIDE is used as an antineoplastic agent, which means it has the potential to inhibit or prevent the growth and spread of cancer cells.
Used in Depot Intramuscular Injections:
FLUPHENAZINE HYDROCHLORIDE is used in long-acting preparations for depot intramuscular injections, such as the enanthate and decanoate esters. These formulations are particularly useful for treating psychotic patients who may not adhere to their medication regimen or are prone to frequent relapses, as they provide a sustained release of the drug over an extended period.
Originator
Prolixin, Squibb ,US ,1959
Manufacturing Process
A suspension of 69.0 grams of 2-trifluoromethylphenothiazine in 1 liter of toluene with 10.9 grams of sodium amide is heated at reflux with high speed stirring for 15 minutes. A solution of 54.1 grams of 1-formyl-4-(3'chloropropyl)-piperazine, [prepared by formylating 1-(3'-hydroxypropyl)piperazine by refluxing in an excess of methyl formate, purifying the 1-formyl4-(3'-hydroxypropyl)-piperazine by vacuum distillation, reacting this compound with an excess of thionyl chloride at reflux and isolating the desired 1-formyl-4(3'-chloropropyl)-piperazine by neutralization with sodium carbonate solution followed by distillation] in 200 ml of toluene is added. The reflux period is continued for 4 hours. The cooled reaction mixture is treated with 200 ml of water. The organic layer is extracted twice with dilute hydrochloric acid. The acid extracts are made basic with ammonia and extracted with benzene. The volatiles are taken off in vacuo at the steam bath to leave a dark brown oil which is 10-[3'-(N-formylpiperazinyl)-propyl]-2trifluoromethylphenothiazine. It can be distilled at 260°C at 10 microns, or used directly without distillation if desired.A solution of 103.5 grams of 10-[3'-(N-formylpiperazinyl)-propyl]-2trifluoromethylphenothiazine in 400 ml of ethanol and 218 ml of water containing 26 ml of 40% sodium hydroxide solution is heated at reflux for 2 hours. The alcohol is taken off in vacuo on the steam bath. The residue is swirled with benzene and water. The dried benzene layer is evaporated in vacuo. The residue is vacuum distilled to give a viscous, yellow oil, 10(3'piperazinylpropyl)-2-trifluoromethylphenothiazine, distilling at 210° to235°C at 0.5 to 0.6 mm.A suspension of 14.0 grams of 10-(3'-piperazinylpropyl)-2trifluoromethylphenothiazine, 6.4 grams of β-bromoethyl acetate and 2.6 grams of potassium carbonate in 100 ml of toluene is stirred at reflux for 16 hours. Water (50 ml) is added to the cooled mixture. The organic layer is extracted into dilute hydrochloric acid. After neutralizing the extracts and taking the separated base up in benzene, a viscous, yellow residue is obtained by evaporating the organic solvent in vacuo. This oil is chromatographed on alumina. The purified fraction of 7.7 grams of 10-[3'-(Nacetoxyethylpiperazinyl)-propyl] -2-trifluoromethylphenothiazine is taken up in ethyl acetate and mixed with 25 ml of alcoholic hydrogen chloride. Concentration in vacuo separates white crystals of the dihydrochloride salt, MP 225° to 227°C.A solution of 1.0 gram of 10-[3'-(N-acetoxyethylpiperazinyl)-propyl]-2trifluoromethylphenothiazine in 25 ml of 1 N hydrochloric acid is heated at reflux briefly. Neutralization with dilute sodium carbonate solution and extraction with benzene gives the oily base, 10-[3'-(N-βhydroxyethylpiperazinyl)-propyl]-2-trifluoromethylphenothiazine. The base is reacted with an excess of an alcoholic hydrogen chloride solution. Trituration with ether separates crystals of the dihydrochloride salt, MP 224° to 226°C, (from US Patent 3,058,979).
Therapeutic Function
Tranquilizer
Biological Activity
ec50: 1.24 μmfluphenazine is a dopamine d1 and d2 receptor inhibitor.dopamine d1 and d2 receptor immunohistochemistry has been used to study the structure of the adult rat arcuate-median eminence complex, particularly in relation to the tubero-infundibular dopamine neurons.
Biochem/physiol Actions
D1/D2 dopamine receptor antagonist; phenothiazine antipsychotic; H1 histamine receptor antagonist.
in vitro
previous study showed that both phenothiazines of fluphenazine and perphenazine induced concentration-dependent loss in cell viability with ec50s to be 1.24 and 2.76 μm for fluphenazine and perphenazine, respectively. moreover, fluphenazine at 1.0 μm and perphenazine at 1.0 and 3.0 μm could inhibit melanogenesis and decrease the content of microphthalmia-associated transcription factor. in addition, both fluphenazine and perphenazine at higher concentrations caused depletion of melanocytes antioxidant status, indicating oxidative stress induction [1].
in vivo
systemic fluphenazine could effectively attenuate mechanical allodynia in rat neuropathic pain models at 0.03-0.3 mg/kg doses, which approximated those used in rodent models of psychosis. for antiallodynic effect, fluphenazine was able to effectively suppress the ectopic discharges in injured afferent fibers without affecting the propagation of action potentials in an ex-vivo drg-nerve preparation from cci rats [2].
references
[1] otreba m et al. fluphenazine and perphenazine impact on melanogenesis and antioxidant enzymes activity in normal human melanocytes. acta poloniae pharmaceutica-drug research, july-august 2016, 73(4):903-911.[2] dong xw,jia y,lu sx,zhou x,cohen-williams m,hodgson r,li h,priestley t. the antipsychotic drug, fluphenazine, effectively reverses mechanical allodynia in rat models of neuropathic pain. psychopharmacology (berl). 2008 jan;195(4):559-68.
Check Digit Verification of cas no
The CAS Registry Mumber 146-56-5 includes 6 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 3 digits, 1,4 and 6 respectively; the second part has 2 digits, 5 and 6 respectively.
Calculate Digit Verification of CAS Registry Number 146-56:
(5*1)+(4*4)+(3*6)+(2*5)+(1*6)=55
55 % 10 = 5
So 146-56-5 is a valid CAS Registry Number.
InChI:InChI=1/C22H26F3N3OS.ClH/c23-22(24,25)17-6-7-21-19(16-17)28(18-4-1-2-5-20(18)30-21)9-3-8-26-10-12-27(13-11-26)14-15-29;/h1-2,4-7,16,29H,3,8-15H2;1H