1461-15-0 Usage
Description
Fluorexon is a chemical compound with the chemical formula C21H27F3N2O3S and is a derivative of fluorescein. It is characterized by its bright orange-yellow color and its ability to emit strong fluorescence when exposed to light. Fluorexon is known for its high sensitivity and selectivity towards certain ions and molecules, making it a valuable tool in various applications.
Uses
Used in Biological Systems:
Fluorexon is used as a fluorimetric indicator for the determination of calcium (Ca) in biological systems. It is particularly useful in complexometric titration, where it forms a stable complex with calcium ions, allowing for accurate measurement.
Used in Membrane Integrity Assays:
Due to its non-membrane permeable form, Fluorexon can be used in assays that evaluate the integrity of cell membranes. This property makes it a valuable tool for studying the effects of various substances on cell membrane function and stability.
Used as a Fluorescent Indicator:
Fluorexon is also used as a fluorescent indicator for other ions and molecules, such as fluoride, iron, and mercury. Its high sensitivity and selectivity make it an ideal choice for detecting and quantifying these substances in various solutions.
Used in Medical Imaging:
Fluorexon has potential applications in magnetic resonance imaging (MRI)-guided therapies, such as sonodynamic and nitric oxide combination therapy. Its strong fluorescence allows for better visualization and monitoring of the treatment process.
Chemical Properties:
Fluorexon is characterized by its orange-yellow color and crystalline structure. Its chemical properties make it a versatile compound with a wide range of applications in various fields, including biology, medicine, and environmental science.
Biological Activity
Calcein is a fluorescent dye and self-extinguishing probe that can be used as an indicator of lipid vesicle leakage, as well as a complex indicator for EDTA calcium titration and fluorescence determination of calcium.
in vitro
Calcein accumulates intracellularly in coelomocytes incubated in coelomic fluid or ISO-EDTA. Coelomocytes incubated in CF show an increase in the calcein fluorescence intensity compared to the control.
Purification Methods
Dissolve it in distilled H2O and acidify with dilute HCl to pH 3.5. Filter off the solid acid and wash it well with H2O. Redissolve ca 10g in 300mL H2O containing 12g of NaOAc. Precipitate it again by adding HCl, filtering and washing with H2O. Add the solid to 200mL of EtOH stir for 1hour and filter. Repeat the EtOH wash and dry the bright yellow solid in a vacuum. This acid decomposes on heating at ca 180o. See below for the preparation of the Na salt. [Diehl & Ellingboe Anal Chem 28 882 1956]. Altenatively dissolve it in H2O and acidify with 3N HCl to pH 3.5. Collect the solid and wash it with H2O. The air-dried precipitate is extracted with 70% aqueous EtOH, filtered hot and cooled slowly. Fine yellow needles of the acid crystallise out; they are filtered off and dissolved in the minimum quantity of 0.01N NaOH and re-precipitated by adding N HCl to pH 3.5. It is then recrystallised from 70% aqueous EtOH (3x). The final product (acid) is dried at 80o in a vacuum for 24hours, m >300o(dec). It contains one molecule of water per molecule of acid (C30H36N4O13.H2O). The product is pure as revealed by electrophoresis at pH 5.6 and 8.6, and by TLC in i-BuOH/i-PrOH/AcOH/H2O (60:60:5:5 by volume) or i-PrOH or pH 8.0 borate buffer. [Wallach et al. Anal Chem 31 456 1959.] The Na salt is prepared by dissolving the pure acid in H2O containing 2 mols of NaOH per mol of acid reagent and lyophilising. It complexes with Ca and Mg ions. [Beilstein 19 III/IV 4338.]
Check Digit Verification of cas no
The CAS Registry Mumber 1461-15-0 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 1,4,6 and 1 respectively; the second part has 2 digits, 1 and 5 respectively.
Calculate Digit Verification of CAS Registry Number 1461-15:
(6*1)+(5*4)+(4*6)+(3*1)+(2*1)+(1*5)=60
60 % 10 = 0
So 1461-15-0 is a valid CAS Registry Number.
InChI:InChI=1/C30H26N2O13/c33-21-7-23-19(5-15(21)9-31(11-25(35)36)12-26(37)38)30(18-4-2-1-3-17(18)29(43)45-30)20-6-16(22(34)8-24(20)44-23)10-32(13-27(39)40)14-28(41)42/h1-8,33-34H,9-14H2,(H,35,36)(H,37,38)(H,39,40)(H,41,42)
