15265-26-6 Usage
Description
Phenolphthalein glucuronic acid, also known as Phenolphthalein β-D-Glucuronide, is a glucuronide conjugate derived from the reaction between phenolphthalein and glucuronic acid. It is characterized by its high water solubility and is widely utilized in various applications due to its unique properties.
Uses
Used in Enzyme Assays:
Phenolphthalein glucuronic acid is used as a substrate for β-glucuronidase tests, specifically for liver and bacterial enzymes. Its high water solubility makes it an ideal candidate for these assays, allowing for accurate and efficient measurements of enzyme activity.
Used in Pharmaceutical and Biomedical Research:
Phenolphthalein glucuronic acid is employed in the development and testing of new drugs and therapies, particularly in the fields of liver and bacterial enzyme research. Its unique properties and high water solubility make it a valuable tool for understanding enzyme function and developing targeted treatments for various diseases and conditions.
Biochem/physiol Actions
Phenolphthalein β-D-glucuronide is a chromogenic substrate for β-glucuronidase enzyme and the resultant colored product of the reaction is measured at 552 nm.
Check Digit Verification of cas no
The CAS Registry Mumber 15265-26-6 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 1,5,2,6 and 5 respectively; the second part has 2 digits, 2 and 6 respectively.
Calculate Digit Verification of CAS Registry Number 15265-26:
(7*1)+(6*5)+(5*2)+(4*6)+(3*5)+(2*2)+(1*6)=96
96 % 10 = 6
So 15265-26-6 is a valid CAS Registry Number.
InChI:InChI=1/C26H22O10.Na/c27-15-9-5-13(6-10-15)26(18-4-2-1-3-17(18)24(33)36-26)14-7-11-16(12-8-14)34-25-21(30)19(28)20(29)22(35-25)23(31)32;/h1-12,19-22,25,27-30H,(H,31,32);/q;+1/p-1/t19-,20-,21+,22-,25+,26?;/m0./s1
15265-26-6Relevant articles and documents
One-pot method for preparing substrate for detecting beta-D-glucuronidase
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Paragraph 0016-0019, (2020/06/17)
The invention discloses a one-pot method for preparing a substrate for detecting beta-D-glucuronidase. According to the method, specifically a compound with a structure of a formula (I) and acetobromo-alpha-D-glucuronic acid methyl ester are used as raw materials, the raw materials are subjected to a reaction for 8-24 h in the presence of a polar solvent and a coupling reagent, then an alkaline reagent is added, a reaction is carried out at room temperature for 4-8 h, then an acidic reagent is added to adjust pH to 2-3, and the substrate for detecting beta-D-glucuronidase is prepared through post-treatment. The method uses phenolphthalein with a substituted or un-substituted phenol structure, and acetobromo-alpha-D-glucuronic acid methyl ester as reactants to prepare the substrate for detecting beta-D-glucuronidase, reaction steps and operation are very simple and convenient, the process complexity is reduced, and thereby the expanded production is easy.
