157134-53-7 Usage
Description
DI-8-ANEPPS is a potentiometric fluorescent dye that is utilized for noninvasive measurement of transmembrane voltage. It exhibits non-fluorescent properties in water, but upon binding to the lipid bilayer, it becomes strongly fluorescent with an excitation/emission maximum of approximately 467/631 nm. The fluorescence intensity of DI-8-ANEPPS is directly proportional to the changes in transmembrane voltage, making it particularly suitable for measuring significant alterations in membrane voltage, such as those occurring in nonexcitable cells exposed to external electric fields or in action potentials of excitable cells. Additionally, DI-8-ANEPPS can function as a cell membrane marker due to its staining properties.
Uses
1. Used in Biomedical Research:
DI-8-ANEPPS is used as a potentiometric fluorescent dye for monitoring transmembrane voltage changes in cells. Its application is particularly beneficial in studying the effects of external electric fields on nonexcitable cells and action potentials in excitable cells.
2. Used in Cell Membrane Marking:
DI-8-ANEPPS is used as a cell membrane marker due to its ability to stain the membrane. This property allows researchers to visualize and track cell membranes in various experimental setups.
3. Used in Long-term Membrane Potential Studies:
DI-8-ANEPPS is employed as a fast-responding membrane potential dye, making it more suitable for long-term studies than other dyes like di-4-ANEPPS. Its better retention in the outer leaflet of plasma membranes and reduced susceptibility to cellular internalization due to the presence of a sulfonate group contribute to its effectiveness in these studies.
4. Used in Dyes and Metabolites Research:
DI-8-ANEPPS is utilized in the study of dyes and metabolites, particularly in understanding their interaction with lipophilic environments such as cell membranes, which leads to a significant increase in fluorescence upon binding.
Check Digit Verification of cas no
The CAS Registry Mumber 157134-53-7 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,5,7,1,3 and 4 respectively; the second part has 2 digits, 5 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 157134-53:
(8*1)+(7*5)+(6*7)+(5*1)+(4*3)+(3*4)+(2*5)+(1*3)=127
127 % 10 = 7
So 157134-53-7 is a valid CAS Registry Number.
InChI:InChI=1/C36H52N2O3S/c1-3-5-7-9-11-13-25-38(26-14-12-10-8-6-4-2)36-21-20-34-30-33(18-19-35(34)31-36)17-16-32-22-27-37(28-23-32)24-15-29-42(39,40)41/h16-23,27-28,30-31H,3-15,24-26,29H2,1-2H3
