163795-75-3

Basic information

• Product Name: SYBR(R) GREEN I NUCLEIC ACID GEL STAIN
• Synonyms: Green-DNA Dye,SYBR Green I;SDNA-Nucleic acids stain dye;SYBR(R) GREEN I;SYBR(R) GREEN I NUCLEI ACID GEL STAIN;SYBR(R) GREEN I NUCLEIC ACID GEL STAIN;SYBR GREEN I;SYBR?Green I nucleic acid gel stain;SYBR(R) GREEN I NUCLEIC ACID GEL STAIN, 500 UL
• CAS NO: 163795-75-3
• Molecular Formula: C32H39N4S+
• Molecular Weight: 511.74386
• EINECS: 999-999-2
• Mol File: 163795-75-3.mol
• Article Data: 0

Chemical Properties

• Flash Point: 94℃
• Appearance: /
• Storage Temp.: −20°C
• CAS DataBase Reference: SYBR(R) GREEN I NUCLEIC ACID GEL STAIN(CAS DataBase Reference)
• NIST Chemistry Reference: SYBR(R) GREEN I NUCLEIC ACID GEL STAIN(163795-75-3)
• EPA Substance Registry System: SYBR(R) GREEN I NUCLEIC ACID GEL STAIN(163795-75-3)

Safety Data

• WGK Germany: 1
• F: 3
• Hazardous Substances Data: 163795-75-3(Hazardous Substances Data)

Usage

Description

SYBR(R) GREEN I NUCLEIC ACID GEL STAIN is an asymmetrical cyanine dye used for detecting nucleic acids. It consists of a N-alkylated benzothiazolium or benzoxazolium ring system, joined by a monomethine bridge to a pyridinium or quinolinium ring system. This cationic unsymmetrical cyanine dye binds to the minor groove of double-stranded DNA (dsDNA) and is excited at a wavelength of 480 nm, with a peak fluorescence emission of 520 nm.
Usage:
Used in Molecular Biology:
SYBR(R) GREEN I NUCLEIC ACID GEL STAIN is used as a nucleic acid stain for detecting and analyzing nucleic acids in molecular biology research. Its high sensitivity and specificity make it a valuable tool for various applications.
Used in Real-time PCR Quantification:
In the field of real-time PCR, SYBR(R) GREEN I NUCLEIC ACID GEL STAIN is used as a quantification agent. It binds to the double-stranded DNA generated during PCR, allowing for the monitoring of amplification and quantification of the target nucleic acid sequence.
Used in Flow Cytometry:
SYBR(R) GREEN I NUCLEIC ACID GEL STAIN is also utilized in flow cytometry as a fluorescent marker for detecting and analyzing nucleic acids in cell populations. This enables researchers to study cell characteristics and functions based on their nucleic acid content.

InChI:InChI=1/C32H37N4S/c1-5-20-35(22-13-21-33(2)3)31-23-25(24-32-34(4)29-18-11-12-19-30(29)37-32)27-16-9-10-17-28(27)36(31)26-14-7-6-8-15-26/h6-12,14-19,23-24H,5,13,20-22H2,1-4H3/q+1