189232-42-6 Usage
Biological Activity
a highly conserved array of mechanisms regulating cyclin-dependent kinases (cdk), which govern the timing of cell cycle progression and division, has developed in eukaryotes. cdk inhibitors have been shown to play an key regulatory role in cell proliferation, differentiation, senescence, as well as programmed death.
in vitro
bohemine was applied to cultures of mouse hybridoma cells in order to evaluate its capacity of cell growth and the production of monoclonal antibody. results showed that addition of bohemine at concentrations in the range of 1-10 μm led to a short-term arrest of growth and monoclonal antibody production. such short-term suppression of cell functions was followed by a significant temporary increase of specific growth rate and specific production rate. in semicontinuous cultures, the steady-state viable cell density values showed a certain stimulation of cell growth in the presence of bohemine at micromolar concentrations, and growth inhibition at 10 and 30 μm [1].
in vivo
in mice, bohemine was found to be rapidly and completely metabolized and disappeared from circulation during the first 60 min following i.v. administration. moreover, the metabolites were eliminated by the hepatobiliary tract and also by renal excretion [2].
IC 50
1 μm for cyclin-dependent kinase
references
[1] franek f,strnad m,havlícek l,siglerová v,fismolová i,eckschlager t. diverse effects of the cyclin-dependent kinase inhibitor bohemine: concentration- and time-dependent suppression or stimulation of hybridoma culture. cytotechnology.2001 jul;36(1-3):117-23. [2] chmela z,vesel j,lemr k,rypka m,hanus j,havlícek l,krystof v,michnová l,fuksová k,lukes j. in vivo metabolism of 2,6,9-trisubstituted purine-derived cyclin-dependent kinase inhibitor bohemine in mice: glucosidation as the principal metabolic route. drug metab dispos.2001 mar;29(3):326-34.
Check Digit Verification of cas no
The CAS Registry Mumber 189232-42-6 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,8,9,2,3 and 2 respectively; the second part has 2 digits, 4 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 189232-42:
(8*1)+(7*8)+(6*9)+(5*2)+(4*3)+(3*2)+(2*4)+(1*2)=156
156 % 10 = 6
So 189232-42-6 is a valid CAS Registry Number.
189232-42-6Relevant articles and documents
Microwave-assisted solid-phase synthesis (MASS) of 2,6,9-trisubstituted purines
Austin, Richard E.,Okonya, John F.,Bond, Daniel R.S.,Al-Obeidi, Fahad
, p. 6169 - 6171 (2002)
In an attempt to discover a high-throughput method for the synthesis of 2,6,9-trisubstituted purines, it was found that microwave irradiation was beneficial in accelerating the nucleophilic displacement of halogens by amines at the C-2 position of the purine nucleus. A method for microwave-assisted solid-phase synthesis (MASS) of 2,6,9-trisubstituted purines is presented.
Facile and efficient access to 2,6,9-tri-substituted purines through sequential N9, N2 Mitsunobu reactions
Fletcher, Steven,Shahani, Vijay M.,Gunning, Patrick T.
scheme or table, p. 4258 - 4261 (2009/10/26)
A facile, efficient and mild synthesis of 2,6,9-tri-substituted purines is presented, starting from commercially available 2-amino-6-chloropurine, which employs sequential N9 then N2 Mitsunobu reactions as key steps. Importantly, our synthetic approach to
