190834-10-7Relevant articles and documents
Synthesis of oligonucleotides containing 3'-alkyl amines using isobutyryl protected deoxyadenosine phosphoramidite
McMinn, Dustin L.,Greenberg, Marc M.
, p. 3123 - 3126 (1997)
Oligonucleotides were synthesized using the N-isobutyryldeoxyadenosine protected β-cyanoethyl phosphoramidite, 9. The oligonucleotides that were synthesized using 9 can be deprotected using concentrated NH4OH at 55°C in 2 h. No depurination was detected via anion exchange HPLC. The N-isobutyryl protected deoxyadenosine did not undergo transamidation with nucleobase and phosphate protected oligonucleotides containing primary alkyl amines, and will be useful in the synthesis of bioconjugates using protected biopolymers.
