19135-17-2 Usage
Description
NPGB, or 4-Nitrophenyl 4-guanidinobenzoate hydrochloride, is a trypsin substrate that is more water-soluble than 4-methylumbelliferyl 4-guanidinobenzoate and is preferred in spectrophotometric assays. It is a compound used in various experimental procedures due to its properties and reactivity.
Uses
Used in Biochemical Research:
NPGB is used as a substrate for trypsin in active site titration experiments, allowing researchers to study the activity and properties of trypsin, a serine protease enzyme.
Used in Mosquito Egg Treatment:
In the interplasmid transposition assay, NPGB is used for pre-treating mosquito eggs. It serves as a component of the isotonic buffer, which is used to moisten filter paper for mosquito embryo collection, ensuring the proper environment for the eggs during the experiment.
Used in Spectrophotometric Assays:
Due to its increased water solubility, NPGB is favored in spectrophotometric assays, providing a more reliable and accurate measurement of enzyme activity and other biochemical processes.
Biochem/physiol Actions
4-Nitrophenyl 4-guanidinobenzoate hydrochloride (pNPGB) inhibits phenoloxidase enzyme and delays embryo chorionic membrane hardening.
Check Digit Verification of cas no
The CAS Registry Mumber 19135-17-2 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 1,9,1,3 and 5 respectively; the second part has 2 digits, 1 and 7 respectively.
Calculate Digit Verification of CAS Registry Number 19135-17:
(7*1)+(6*9)+(5*1)+(4*3)+(3*5)+(2*1)+(1*7)=102
102 % 10 = 2
So 19135-17-2 is a valid CAS Registry Number.
InChI:InChI=1/C14H12N4O4.ClH/c15-14(16)17-10-3-1-9(2-4-10)13(19)22-12-7-5-11(6-8-12)18(20)21;/h1-8H,(H4,15,16,17);1H
