193884-53-6

Basic information

• Product Name: APS-5
• Synonyms: APS-5;[(4-Chlorophenyl)thio](10-methyl-9(10H)-acridinylidene)-methanol 1-(dihydrogen phosphate) disodium salt (1:2);Lumigen APS 5;Disodium [(4-chlorophenyl)sulfanyl](10-methyl-9(10H)-acridinylidene)methyl phosphate;[(4-chlorophenyl)thio](10-methyl-9(10H)-acridinylidene)methanol 1-(dihydrogen phosphate) disodium salt
• CAS NO: 193884-53-6
• Molecular Formula: C21H18ClNNaO4PS
• EINECS: -0
• Mol File: 193884-53-6.mol
• Article Data: 0

Chemical Properties

• Appearance: /
• CAS DataBase Reference: APS-5(CAS DataBase Reference)
• NIST Chemistry Reference: APS-5(193884-53-6)
• EPA Substance Registry System: APS-5(193884-53-6)

Safety Data

• Hazardous Substances Data: 193884-53-6(Hazardous Substances Data)

Usage

Description

APS-5, also known as Lumigen APS-5, is a proprietary acridan-based chemiluminescent substrate specifically designed for the detection of alkaline phosphatase (AP) conjugated molecules in enzyme-linked immunosorbent assay (ELISA). It is based on the 9,10-dihydroacridine structure, which allows for continuous, stable, and efficient light emission under the action of AP. This unique acridan chemistry provides temperature-insensitive light output and a more rapid peak intensity compared to dioxetane-based AP substrates.

Uses

Used in Diagnostics and Research:
APS-5 is used as a chemiluminescent substrate for the detection of compounds with alkaline phosphatase (AP) conjugation in ELISA. The 9,10-dihydroacridine structure in APS-5 enables stable and efficient light emission, making it highly suitable for determining phosphatase activity in solutions and phosphatase-linked immunoassays.
Used in Pharmaceutical and Biotechnology Industries:
In the pharmaceutical and biotechnology industries, APS-5 is used as a detection tool for the development and testing of new drugs, vaccines, and diagnostic tools that involve alkaline phosphatase conjugation. Its temperature-insensitive light output and rapid peak intensity make it an ideal choice for research and development purposes.
Used in Quality Control and Validation:
APS-5 is also utilized in quality control and validation processes to ensure the accuracy and reliability of alkaline phosphatase-based assays. Its stable and efficient light emission properties make it a valuable tool for monitoring the performance of these assays and ensuring their consistency across various applications.

Mechanism of action

Lumigen APS-5 utilizes a unique technology for the chemiluminescent detection of alkaline phosphatase conjugates which combine excellent sensitivity with ease of use. Reaction of the acridan substrate with an AP label rapidly produces sustained high-intensity chemiluminescence. Lumigen APS-5 is ideally suited for solution assays of phosphatase activity and for phosphatase-linked immunoassays.

Advantages

1.High sensitivity -- protein markers less than 5pg can be detected;2. High luminescence intensity -- peak luminescence can be achieved in a short time;3. Continuous and stable luminescence - the luminescence process is not affected by temperature within 25-35 days and does not need temperature control.