198561-70-5Relevant articles and documents
Synthesis of chromogenic substrates of α-amylases on a cyclodextrin basis
Farkas, Erzsebet,Janossy, Lorant,Harangi, Janos,Kandra, Lili,Liptak, Andras
, p. 407 - 415 (2007/10/03)
One-pot acetylation and subsequent partial acetolysis of α-, β-and γ- cyclodextrins resulted in crystalline peracetylated malto-hexaose, -heptaose, and -octaose, respectively. Prolonged acetolysis of β-cyclodextrin gave a mixture of acetylated maltooligosaccharides, from which peracetylated malto- triose, -tetraose, and -pentaose were isolated. The acetylated oligosaccharides were converted into α-acetobromo derivatives, and then transformed into 4-nitrophenyl and 2-chloro-4-nitrophenyl β-glycosides. From the 4-nitrophenyl glycosides 4,6-O-benzylidene derivatives were prepared, which were used together with the free glycosides as substrates of porcine pancreatic α-amylase.