2150-48-3 Usage
Description
CI 45010, also known as Pyronin B, is a synthetic dye derived from fluorescein and is commonly used in various staining and coloration methods. It exhibits strong fluorescence properties and is characterized by its bright red coloration, making it a valuable tool in the field of histology and cytology.
Uses
Used in Histology and Cytology:
CI 45010 is used as a staining agent for the coloration of nucleic acids, particularly in the methyl green-pyronin method. This method allows for the differentiation and visualization of DNA and RNA within cells, making it a crucial tool in the study of cellular processes and structures.
Used in the Methyl Green-Pyronin Method:
CI 45010 is used as a component in the methyl green-pyronin staining method for differential coloration of nucleic acids. In this method, methyl green binds to DNA, while Pyronin B (CI 45010) binds to RNA, resulting in distinct coloration that aids in the identification and analysis of these nucleic acids.
Used in Dyes and Metabolites:
CI 45010 is also utilized in the production of various dyes and metabolites, where its unique properties contribute to the development of new compounds with potential applications in various industries, including pharmaceuticals, textiles, and cosmetics.
Purification Methods
Recrystallise it from EtOH. It forms an Fe stain. [Beilstein 18/10 V 182.]
Check Digit Verification of cas no
The CAS Registry Mumber 2150-48-3 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 2,1,5 and 0 respectively; the second part has 2 digits, 4 and 8 respectively.
Calculate Digit Verification of CAS Registry Number 2150-48:
(6*2)+(5*1)+(4*5)+(3*0)+(2*4)+(1*8)=53
53 % 10 = 3
So 2150-48-3 is a valid CAS Registry Number.
InChI:InChI=1/C21H27N2O.4ClH.Fe/c1-5-22(6-2)18-11-9-16-13-17-10-12-19(23(7-3)8-4)15-21(17)24-20(16)14-18;;;;;/h9-15H,5-8H2,1-4H3;4*1H;/q+1;;;;;+3/p-4
2150-48-3Relevant articles and documents
One-pot synthesis of N, N, N, N-tetraethyl-9H-xanthene-3,6-diamine and its conversion to pyronine B
Han, Shulei,Chen, Yi
, p. 59 - 62 (2013)
N, N, N, N-tetraethyl-9H-xanthene-3,6-diamine (9H-xanthene) was prepared by one-pot reaction of 3-diethylaminophenol with (CH3O)3CH using pyridinium para-toluenesulfonate (PPTS) as catalyst. It was found that 9H-xanthene is photo-active and could be efficiently photo-converted to pyronine B. Treatment of 9H-xanthene with acid (HCl) produced protonated 9H-xanthene (9H-xanthene·HCl), and 9H-xanthene·HCl was spontaneously converted to pyronine B in aqueous solution. Besides, both distinct color change and turn on fluorescence were observed during the conversion. These findings not only provide a new and simple approach to the preparation of 9H-xanthene and pyronine B but also benefit the applications such as indicator and probe.
A concise colorimetric and fluorimetric probe for sarin related threats designed via the covalent-assembly approach
Lei, Zuhai,Yang, Youjun
, p. 6594 - 6597 (2014/05/20)
A turn-on signal from zero background allows sensitive detection of a weak signal and is highly desired. The covalent-assembly probe design principle is powerful in this regard. Herein, we report an embodiment of this principle (NA570) for detection of Sa
