216699-36-4

Basic information

• Product Name: 6-ROX, SE
• Synonyms: 6-CXR SE;6-ROX SE, 6-CXR SE;6-ROX, SE [6-Carboxy-X-rhodaMine, suciniMidyl ester];1H,5H,11H,15H-Xantheno[2,3,4-ij:5,6,7-i'j']diquinolizin-18-ium,9-[2-carboxy-5-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-2,3,6,7,12,13,16,17-octahydro-,inner salt;6-CARBOXY-X-RHODAMINE N-SUCCINIMIDYL ESTER;6-CARBOXY-X-RHODAMINE, SUCCINIMIDYL ESTER;6-ROX, SE;6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester] *CAS#: 216699-36-4*
• CAS NO: 216699-36-4
• Molecular Formula: C₃₇H₃₃N₃O₇
• Molecular Weight: 631.68
• Product Categories: Nucleic Acid Stains For MicroscopyFluorescent Probes, Labels, Particles and Stains;Biochemicals and Reagents;DetectionFluorescent Stains;Fluorescent Labels;Fluorescent Probes, Labels, Particles and Stains;Nucleic Acid Stains;Rhodamine and Derivatives
• Mol File: 216699-36-4.mol
• Article Data: 1

Chemical Properties

• Boiling Point: °Cat760mmHg
• Flash Point: °C
• Appearance: /
• Density: g/cm³
• Storage Temp.: −20°C
• Solubility: DMF: soluble
• CAS DataBase Reference: 6-ROX, SE(CAS DataBase Reference)
• NIST Chemistry Reference: 6-ROX, SE(216699-36-4)
• EPA Substance Registry System: 6-ROX, SE(216699-36-4)

Safety Data

• Hazard Codes: Xi
• Statements: 36/37/38
• Safety Statements: 26-36
• WGK Germany: 3
• F: 8-10-21
• Hazardous Substances Data: 216699-36-4(Hazardous Substances Data)

Usage

Description

6-ROX, SE, also known as 6-Carboxy-X-rhodamine N-succinimidyl ester, is a fluorescent probe and a rhodamine X derivative. It is an amine-reactive fluorescent probe used for labeling oligonucleotides and preparing labeled primers for real-time PCR applications.

Uses

Used in Molecular Biology:
6-ROX, SE is used as an amine-reactive fluorescent probe for labeling oligonucleotides, which allows for the detection and analysis of specific DNA or RNA sequences in various molecular biology techniques.
Used in Real-Time PCR:
6-ROX, SE is used as a labeling agent for the preparation of labeled primers, which are essential for real-time PCR. This enables the monitoring of PCR amplification in real-time, providing accurate quantification of target nucleic acid sequences.
Used in Fluorescence Detection:
As a rhodamine X derivative, 6-ROX, SE is used in fluorescence detection methods due to its strong fluorescence properties. This makes it suitable for various applications, such as fluorescence microscopy, flow cytometry, and other fluorescence-based assays.

Upstream product

• 552-30-7 trimellitic Anhydride 
• 41175-50-2 8-hydroxyjulolidine 
• 74124-79-1 di(succinimido) carbonate 
• 1233524-42-9 7-(tert-butyldimethylsilanyloxy)-1,2,3,4-tetrahydroquinoline 

Downstream Products

• 857285-12-2 C₄₂H₄₀N₄O₇ 

Relevant articles and documents

Hydroxylated Fluorescent Dyes for Live-Cell Labeling: Synthesis, Spectra and Super-Resolution STED

Hydroxylated rhodamines, carbopyronines, silico- and germanorhodamines with absorption maxima in the range of 530–640 nm were prepared and applied in specific labeling of living cells. The direct and high-yielding entry to germa- and silaxanthones tolerates the presence of protected heteroatoms and may be considered for the syntheses of various sila- and germafluoresceins, as well as -rhodols. Application in stimulated emission depletion (STED) fluorescence microscopy revealed a resolution of 50–75 nm in one- and two-color imaging of vimentin-HaloTag fused protein and native tubulin. The established structure–property relationships allow for prediction of the spectral properties and the positions of spirolactone/zwitterion equilibria for the new analogues of rhodamines, carbo-, silico-, and germanorhodamines using simple additive schemes.