229014-55-5Relevant articles and documents
Baclofen artificial hapten, artificial antigen as well as preparation method and application thereof
-
Paragraph 0050-0054; 0101-0107; 0110-0113; 0117-0120, (2020/11/10)
The invention discloses a baclofen artificial hapten, an artificial antigen as well as a preparation method and application thereof. The molecular structural formula of the baclofen artificial haptenis represented by a formula (I), and the molecular structural formula of the baclofen artificial antigen is represented by a formula (II). According to the baclofen artificial hapten, the characteristic structure of baclofen is reserved to the maximum extent, and the baclofen artificial hapten has an active group capable of being coupled with carrier protein and can be used as an antigen determinant; the further prepared baclofen artificial antigen can be used for immunizing to obtain an anti-baclofen antibody with high affinity, high sensitivity and strong specificity, the titer of immune serum obtained by immunizing New Zealand white rabbits is up to 1:80000, and the antibody can be used for carrying out rapid and accurate immunodetection and immunoassay on baclofen.