23983-07-5

Basic information

• Product Name: (2E)-2-(4-hydroxybenzylidene)[1,3]thiazolo[3,2-a]benzimidazol-3(2H)-one
• Synonyms: thiazolo[3,2-a]benzimidazol-3(2H)-one, 2-[(4-hydroxyphenyl)methylene]-, (2E)-
• CAS NO: 23983-07-5
• Molecular Formula: C₁₆H₁₀N₂O₂S
• Molecular Weight: 294.3278
• Mol File: 23983-07-5.mol
• Article Data: 1

Chemical Properties

• Boiling Point: 546.6°C at 760 mmHg
• Flash Point: 284.4°C
• Density: 1.46g/cm³
• Vapor Pressure: 1.47E-12mmHg at 25°C
• Refractive Index: 1.76
• CAS DataBase Reference: (2E)-2-(4-hydroxybenzylidene)[1,3]thiazolo[3,2-a]benzimidazol-3(2H)-one(CAS DataBase Reference)
• NIST Chemistry Reference: (2E)-2-(4-hydroxybenzylidene)[1,3]thiazolo[3,2-a]benzimidazol-3(2H)-one(23983-07-5)
• EPA Substance Registry System: (2E)-2-(4-hydroxybenzylidene)[1,3]thiazolo[3,2-a]benzimidazol-3(2H)-one(23983-07-5)

Safety Data

• Hazardous Substances Data: 23983-07-5(Hazardous Substances Data)

Usage

Description

(2E)-2-(4-hydroxybenzylidene)[1,3]thiazolo[3,2-a]benzimidazol-3(2H)-one, also known as Thioflavin T, is a fluorescent dye that is widely used in the study of protein aggregation and amyloid fibril formation. It is particularly effective in detecting and quantifying beta-amyloid aggregates, which are linked to Alzheimer's disease. Thioflavin T can bind to the cross-beta sheet structure of amyloid fibrils, leading to a significant increase in its fluorescence intensity. This characteristic makes it a valuable tool for visualizing and measuring amyloid formation both in vitro and in vivo. Additionally, Thioflavin T has been employed in various biomedical and biochemical applications, such as the study of prion diseases and other protein misfolding pathologies.

Uses

Used in Alzheimer's Disease Research:
Thioflavin T is used as a diagnostic tool for Alzheimer's disease, specifically for the detection and quantification of beta-amyloid aggregates. Its ability to bind to the cross-beta sheet structure of amyloid fibrils and increase in fluorescence intensity allows for the visualization and measurement of amyloid formation, which is crucial for understanding the progression of the disease and evaluating potential treatments.
Used in Protein Misfolding Pathology Studies:
Thioflavin T is employed as a research tool in the study of prion diseases and other protein misfolding pathologies. Its fluorescence properties enable the investigation of protein aggregation and fibril formation, which are key aspects of these diseases. This helps researchers gain insights into the mechanisms underlying these conditions and develop potential therapeutic strategies.
Used in Biomedical and Biochemical Applications:
Thioflavin T is utilized in various biomedical and biochemical applications, including the study of protein-protein interactions, the development of diagnostic assays, and the investigation of the structural properties of amyloid fibrils. Its versatility as a fluorescent dye makes it a valuable asset in these fields, contributing to advancements in our understanding of protein behavior and its implications for human health.

Upstream product

• 3042-01-1 thiazolo[3,2-a]benzimidazol-3(2H)-one 
• 123-08-0 4-hydroxy-benzaldehyde 
• 134469-07-1 Benzimidazol-2-thiol 
• 3042-00-0 2-(1H-benzimidazol-2-ylthio)acetic acid 

Relevant articles and documents

Synthesis of New 2-Substituted Benzylidenethiazolobenzimidazol-3(2H)-ones and Their Biological Activity

2-Substituted benzylidenethiazolobenzimidazol-3(2H)-ones (I-XIV) have been synthesized and found to possess anticonvulsant activity (0-60percent).These compounds also inhibit rat brain monoamine oxidase (MAO) (33.70-98.30percent) and succinate dehydrogenase (SDH) (25.72-78.95percent) in vitro of concentrations 4E-4 M and 6E-4 M, respectively.Structure-activity relationship has been dicussed.