25709-80-2

Basic information

• Product Name: 4'-Bromo-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
• Synonyms: 4'-Bromo-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
• CAS NO: 25709-80-2
• Molecular Formula: C20H11 Br O5
• Molecular Weight: 411.2
• Mol File: 25709-80-2.mol
• Article Data: 1

Chemical Properties

• Appearance: /
• CAS DataBase Reference: 4'-Bromo-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one(CAS DataBase Reference)
• NIST Chemistry Reference: 4'-Bromo-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one(25709-80-2)
• EPA Substance Registry System: 4'-Bromo-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one(25709-80-2)

Safety Data

• Hazardous Substances Data: 25709-80-2(Hazardous Substances Data)

Upstream product

• 2321-07-5 fluorescein 

Relevant articles and documents

3D-Non-destructive Imaging through Heavy-Metal Eosin Salt Contrast Agents

Conventional histology is a destructive technique based on the evaluation of 2D slices of a 3D biopsy. By using 3D X-ray histology these obstacles can be overcome, but their application is still restricted due to the inherently low attenuation properties of soft tissue. In order to solve this problem, the tissue can be stained before X-ray computed tomography imaging (CT) to enhance the soft tissue X-ray contrast. Evaluation of brominated fluorescein salts revealed a mutual influence of the number of bromine atoms and the cations applied on the achieved contrast enhancement. The dibromo fluorescein barium salt turned out to be the ideal X-ray contrast agent, allowing for 3D imaging and subsequent complementing counterstaining applying standard histological techniques.