27964-99-4

Basic information

• Product Name: D-Lysine homopolymer hydrobromide
• Synonyms: POLY-D-LYSINE HYDROBROMIDE MOL WT*15;POLY-D-LYSINE HYDROBROMIDE MOL WT*70;POLY-D-LYSINE HYDROBROMIDE MOL WT*GR;POLY-D-LYSINE HYDROBROMIDE*MOL WT 30;POLY-D-LYSINE HYDROBROMIDE, MOL WT&;Poly-D-lysine hydrobromide, M.W. 30,000 to 70,000;D-LYS-(D-LYS)N-D-LYS XHBR;POLY-D-LYSINE 70 000 HBR
• CAS NO: 27964-99-4
• Molecular Formula: (C6H12N2O2)n.xHBr
• Molecular Weight: 146.19
• Product Categories: HomopolymersAmino Acids;Polylysine and Polyornithine Attachment Factors;Amino Acids;Polyamino Acids;Homopolymers
• Mol File: 27964-99-4.mol
• Article Data: 0

Chemical Properties

• Appearance: /lyophilized powder
• Storage Temp.: −20°C
• Water Solubility: Soluble in water.
• CAS DataBase Reference: D-Lysine homopolymer hydrobromide(CAS DataBase Reference)
• NIST Chemistry Reference: D-Lysine homopolymer hydrobromide(27964-99-4)
• EPA Substance Registry System: D-Lysine homopolymer hydrobromide(27964-99-4)

Safety Data

• WGK Germany: 3
• F: 10-21
• Hazardous Substances Data: 27964-99-4(Hazardous Substances Data)

Usage

Description

D-Lysine homopolymer hydrobromide is a synthetic polymer derived from the amino acid D-lysine. It is a white to off-white solid and is soluble in water. This polymer has unique properties that make it useful in various applications, particularly in the field of cell culture and neuroscience.

Uses

Used in Animal Cell Culture:
D-Lysine homopolymer hydrobromide is used as a cell culture substratum for promoting cell attachment and growth. It is particularly useful for primary cell cultures and adult neuronal network models, as it provides a stable and supportive surface for cell adhesion.
Used in Rat Cortical Neuron Cultures:
In rat cortical neuron cultures, D-Lysine homopolymer hydrobromide serves as a coating agent to enhance the attachment and growth of neurons. This is crucial for studying neuronal development and function in a controlled in vitro environment.
Used in Cell Culture and siRNA Treatment:
D-Lysine homopolymer hydrobromide is utilized as a coating agent for cell culture plates and coverslips, ensuring better cell adhesion and facilitating the delivery of siRNA for gene silencing experiments.
Used in Primary Cell Cultures and Adult Neuronal Network Model:
This polymer is employed as a coating agent to improve the attachment and growth of primary cells and adult neuronal networks, which are essential for studying the complex interactions and functions of these cells in a controlled environment.
Used in Cortical Astrocyte Culture:
D-Lysine homopolymer hydrobromide is used as a substratum to support the growth and attachment of cortical astrocytes, which play a vital role in maintaining the structural and functional integrity of the central nervous system.
Used in Immunofluorescence Staining:
In immunofluorescence staining, D-Lysine homopolymer hydrobromide is used as a coating agent for glass coverslips, providing a suitable surface for cell attachment and subsequent staining procedures.
Used in NPC Collection and Culture Conditions:
D-Lysine homopolymer hydrobromide is utilized as a coating agent for the collection and culture of neural progenitor cells (NPCs), which are crucial for studying the processes of neurogenesis and neural repair.

Biochem/physiol Actions

This product is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. After absorption to the culture surface, poly-D-lysine increases the number of positively charged cell binding sites.