299953-00-7 Usage
Description
Mirin is an inhibitor of the Mre11/Rad50/Nbs1 (MRN) complex, which functions as a DNA damage sensor and activates the DNA repair enzyme ataxia-telangiectasia mutated kinase (ATM). It inhibits phosphorylation of H2AX induced by DNA containing double-strand DNA breaks (DSBs), indicating decreased ATM activation, with an IC50 value of 66 μM in X. laevis cell-free extracts. Mirin also inhibits the exonuclease activity of Mre11 in a cell-free assay and has been shown to induce cell cycle arrest at the G2 phase and inhibit homology-dependent repair in a concentration-dependent manner.
Uses
Used in Cellular and Molecular Research:
Mirin is used as a small molecule inhibitor for studying the role of the MRN complex in DNA damage response and repair mechanisms. It is particularly useful in understanding the effects of MRN inhibition on cell cycle arrest, DNA repair, and tumor growth.
Used in Cancer Research:
Mirin is used as an Mre11 inhibitor in various cancer cell lines, such as HCT116 wildtype cells, BSC-1 cells infected with simian virus 40, and W12E cells, to study its effects on tumor growth, cell cycle arrest, and DNA repair mechanisms. It is also used in combination with other treatments, such as polyamide-25 (PA-25), to potentiate their effects on cancer cells.
Used in Drug Delivery Systems:
Mirin has been encapsulated in nanoparticles for improved delivery, bioavailability, and therapeutic outcomes in cancer treatment. Nanoparticle-encapsulated mirin has been shown to reduce tumor growth in an LA-N-5 mouse xenograft model, demonstrating its potential as a therapeutic agent in cancer treatment.
Used in Pre-treatment of Cells:
Mirin is used in the pre-treatment of cells before irradiation or other DNA damaging agents to study the effects of MRN inhibition on cellular responses to DNA damage and the potential for enhancing the efficacy of radiation therapy or chemotherapy.
Used in Quantification of Virus Episomes:
Mirin is used in the pre-treatment of cells for quantification of human papilloma virus (HPV) episome levels, providing insights into the role of MRN in virus replication and the potential for targeting MRN in antiviral therapies.
Biochem/physiol Actions
Mirin is an inhibitor of the Mre11-Rad50-Nbs1 complex. It disrupts nuclease activity of Mre11 and thus, the ability to repair DNA double strand breaks.
References
1) Dupre et al. (2008), A forward chemical genetic screen reveals an inhibitor of the Mre11-Rad50-Nbs1 complex; Nat. Chem. Biol., 4 119
Check Digit Verification of cas no
The CAS Registry Mumber 299953-00-7 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 2,9,9,9,5 and 3 respectively; the second part has 2 digits, 0 and 0 respectively.
Calculate Digit Verification of CAS Registry Number 299953-00:
(8*2)+(7*9)+(6*9)+(5*9)+(4*5)+(3*3)+(2*0)+(1*0)=207
207 % 10 = 7
So 299953-00-7 is a valid CAS Registry Number.
299953-00-7Relevant articles and documents
A facile synthesis of 5-arylidene-2-imino-4-thiazolidinones under microwave irradiation
Zhou, Jian-Feng,Sun, Xiao-Jun,Zhu, Feng-Xia,Li, Yan-Lun,Gong, Gui-Xia
experimental part, p. 4182 - 4187 (2009/04/11)
A series of 5-arylidene-2-imino-4-thiazolidinone derivatives were synthesized by the cross-aldol condensation of aromatic aldehydes with 2-imino-4-thiazolidinone in sodium acetate/acetic acid under microwave irradiation. The reactions were completed in 10 min with 63-91% yields, were environmental benign, and had easy workup. Copyright Taylor & Francis Group, LLC.
