303-07-1Relevant articles and documents
Quantitation of the hydroxyl radical adducts of salicylic acid by micellar electrokinetic capillary chromatography: Oxidizing species formed by a Fenton reaction
Tomita,Okuyama,Watanabe,Watanabe
, p. 428 - 433 (1994)
There has been controversy concerning the products formed by a Fenton reaction. We determined the hydroxyl radical (.OH) generated in a Fenton reaction system with no iron chelator using micellar electrokinetic capillary chromatography (MECC). The hydroxyl radical generated in this Fenton system attacked salicylic acid to produce major products of 2,3- and 2,5-dihydroxybenzoic acid (DHB), 2,3-DHB being prominent. Hydroxyl radical scavengers, such as mannitol, ethanol, thiourea and a ferric chelator, Desferal, significantly diminished the peaks for DHBs, showing production of .OH. We compared the MECC method with the electron paramagnetic resonance (EPR) spin trapping technique. The quantity of DHBs obtained by MECC increased dose-dependently up to 1 μM Fe2+ at a fixed concentration of H2O2, whereas that of the spin adduct by EPR showed a bell-shaped curve. This quantitation of .OH adducts by MECC supports the proposal that the oxidizing species formed by a Fenton reaction with no chelator is .OH. The EPR spin trapping method appears to be erroneous, particularly when iron is present at a higher concentration than hydrogen peroxide. The application of this method to the paraquat effect in vitro is demonstrated, and the possibility for analysis of .OH in vivo is also discussed.
HPLC study on Fenton-reaction initiated oxidation of salicylic acid. Biological relevance of the reaction in intestinal biotransformation of salicylic acid
Nyúl, Eszter,Kuzma, Mónika,Mayer, Mátyás,Lakatos, Sándor,Almási, Attila,Perjési, Pál
, p. 1040 - 1051 (2018/10/24)
Fenton-reaction initiated in vitro oxidation and in vivo oxidative biotransformation of salicylic acid was investigated by HPLC-UV-Vis method. By means of the developed high performance liquid chromatography (HPLC) method salicylic acid, catechol, and all the possible monohydroxylated derivatives of salicylic acid can be separated. Fenton oxidations were performed in acidic medium (pH 3.0) with two reagent molar ratios: (1) salicylic acid: iron: hydrogen peroxide 1:3:1 and (2) 1:0.3:1. The incubation samples were analysed at different time points of the reactions. The biological effect of elevated reactive oxygen species concentration on the intestinal metabolism of salicylic acid was investigated by an experimental diabetic rat model. HPLC-MS analysis of the in vitro samples revealed presence of 2,3- and 2,5-dihydroxybenzoic acids. The results give evidence for nonenzyme catalysed intestinal hydroxylation of xenobiotics.
6-sulfonate pyrimidyl salicylate compound and preparation method and application thereof
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Paragraph 0135; 0136; 0137; 0138, (2017/07/19)
The invention relates to the field of herbicides and discloses a 6-sulfonate pyrimidyl salicylate compound and a preparation method and application thereof. The compound has a structure as shown in a formula (1). The method for preparing the 6-sulfonate pyrimidyl salicylate compound comprises the steps of (1) carrying out first contact reaction on the compound as shown in a formula (2) and R2SO2Cl to obtain a compound as shown in a formula (3); and (2) carrying out second contact reaction on the compound as shown in the formula (3) and trifluoroacetic acid. The 6-sulfonate pyrimidyl salicylate compound has a good inhibition effect on acetohydroxyacid synthase, and has significant resistance inhibition effect on weeds which are resistant to an acetohydroxyacid synthase inhibitor herbicide, thereby controlling weed resistance plants caused by acetohydroxyacid synthase. The formulas are as shown in the specification.