31796-55-1

Basic information

• Product Name: H-ALA-PNA HCL
• Synonyms: (S)-2-AMino-N-(4-nitrophenyl)propanaMide hydrochloride;PropanaMide,2-aMino-N-(4-nitrophenyl)-, hydrochloride (1:1), (2S)-;N~1~-(4-nitrophenyl)alaninamide hydrochloride;L-Alanine 4-nitroanilide hydrochloride≥ 99% (HPLC);L-Ala-pNA·HCl;H-L-ALA-PNA HCL;L-2-AMINO-4'-NITROPROPIONANILIDE HYDROCHLORIDE;L-ALANINE-4-NITROANILIDE HYDROCHLORIDE
• CAS NO: 31796-55-1
• Molecular Formula: C9H12ClN3O3
• Molecular Weight: 245.66
• EINECS: 250-811-0
• Product Categories: enzyme;Activity;Chromogenic;Enzyme Substrates;Aminopeptidase;Substrates by Enzyme
• Mol File: 31796-55-1.mol
• Article Data: 0

Chemical Properties

• Boiling Point: 443.4 °C at 760 mmHg
• Flash Point: 221.9 °C
• Appearance: Pale yellow/Powder
• Vapor Pressure: 4.67E-08mmHg at 25°C
• Storage Temp.: −20°C
• Solubility: H2O: 50 mg/mL, clear, yellow
• BRN: 6303731
• CAS DataBase Reference: H-ALA-PNA HCL(CAS DataBase Reference)
• NIST Chemistry Reference: H-ALA-PNA HCL(31796-55-1)
• EPA Substance Registry System: H-ALA-PNA HCL(31796-55-1)

Safety Data

• Safety Statements: 22-24/25
• WGK Germany: 3
• Hazardous Substances Data: 31796-55-1(Hazardous Substances Data)

Usage

Description

H-ALA-PNA HCL, also known as L-Alanine 4-nitroanilide hydrochloride, is an off-white crystalline powder with unique chemical properties. It is a synthetic substrate commonly used in biochemical research and analysis.

Uses

Used in Biochemical Research:
H-ALA-PNA HCL is used as a substrate for determining the activity of aminopeptidase enzymes, which are crucial in various biological processes, including protein digestion and peptide hydrolysis. Its ability to be hydrolyzed by aminopeptidases makes it a valuable tool for studying enzyme kinetics and mechanisms.
Used in Pharmaceutical Industry:
In the pharmaceutical industry, H-ALA-PNA HCL is utilized for the development and evaluation of drugs targeting aminopeptidase enzymes. By measuring the activity of these enzymes in the presence of potential drug candidates, researchers can assess the efficacy and selectivity of new compounds.
Used in Diagnostic Applications:
H-ALA-PNA HCL is employed in diagnostic assays to detect and quantify aminopeptidase activity in biological samples. This can be particularly useful in clinical settings for monitoring certain diseases or conditions where aminopeptidase levels may be altered.
Used in Food Industry:
In the food industry, H-ALA-PNA HCL can be used to study the activity of aminopeptidases involved in the maturation and flavor development of fermented products, such as cheese and wine. Understanding the role of these enzymes can help optimize production processes and improve product quality.

Biochem/physiol Actions

L-Alanine-4-nitroanilide is used as a substrate to determine alanine aminopeptidase (AAP) activity. It is mainly chosen as a substrate, due to its relative specificity, higher hydrolysis rate and better solubility.

InChI:InChI=1/C9H11N3O3.ClH/c1-6(10)9(13)11-7-2-4-8(5-3-7)12(14)15;/h2-6H,10H2,1H3,(H,11,13);1H