333303-11-0Relevant articles and documents
Deep-Red and Near-Infrared Xanthene Dyes for Rapid Live Cell Imaging
Niu, Guangle,Liu, Weimin,Zhou, Bingjiang,Xiao, Hongyan,Zhang, Hongyan,Wu, Jiasheng,Ge, Jiechao,Wang, Pengfei
, p. 7393 - 7399 (2016/09/09)
In this work, two xanthene dyes (H-hNR and TF-hNR) have been synthesized by a convenient and efficient method. These two dyes exhibited deep-red and near-infrared emissions, high fluorescence quantum yields, and good photostability. Their structure-optical properties were investigated by X-ray crystal structure analysis and density functional theory calculations. Live cell imaging data revealed that H-hNR and TF-hNR could rapidly stain both A549 and HeLa cells with low concentrations. The excellent photophysical and imaging properties render them as promising candidates for use in live cell imaging.
Synthesis and fluorescence properties of substituted 7-aminocoumarin-3-carboxylate derivatives
Corrie,Munasinghe,Rettig
, p. 1447 - 1455 (2007/10/03)
4-Trifluoromethyl- or 6-bromo-substituted 7-diethylaminocoumarin-3-carboxamide derivatives 2 and 3, each containing a maleimide have been synthesized as potential fluorescent labeling reagents for thiol groups in proteins and their fluorescence properties have been determined. The 4-trifluoromethyl substituted compound 2 has a significantly greater Stokes shift than the comparable compound lacking this group, but both the new coumarins have low fluorescence quantum yields (φf). When a 4-trifluoromethyl substituent is present, the 3-carboxamide is unusually labile to hydrolysis. Bromination of ethyl 7-diethylaminocoumarin-3-carboxylate 17 gave the 6- and 8-bromo derivatives 18 and 19 respectively, and also the 8-bromo-7-monoethylamino compound 20. φf for the latter compound is 100-fold greater than for its diethylamino analogue 19. Fluorescence lifetime measurements support an interpretation based on the twisted intramolecular charge transfer (TICT) model to explain these large differences in φf.
