3348-03-6 Usage
Description
6-CARBOXYFLUORESCEIN DIACETATE (6-CFDA) is a fluorescent probe that is widely utilized in various applications due to its unique properties. It is a polyanionic compound that can be used to differentiate viable cells from apoptotic cells, as well as to detect changes in intracellular pH. 6-CARBOXYFLUORESCEIN DIACETATE is particularly useful in the field of cell biology and molecular biology, providing valuable insights into cellular processes and functions.
Uses
Used in Cell Biology:
6-CARBOXYFLUORESCEIN DIACETATE is used as a cell viability indicator for distinguishing between viable and apoptotic cells. The expression is: 6-CARBOXYFLUORESCEIN DIACETATE is used as a cell viability indicator for [distinguishing viable cells from apoptotic cells] because [live cells that are not apoptotic accumulate 6-carboxyfluorescein but do not accumulate annexin].
Used in Molecular Biology:
6-Carboxyfluorescein Diacetate is used as a fluorescent probe for detecting changes in intracellular pH. The expression is: 6-CARBOXYFLUORESCEIN DIACETATE is used as a fluorescent probe for [detecting changes in intracellular pH] because [it is a polyanionic compound that can be used to monitor pH variations within cells].
Used in Flow Cytometry:
6-Carboxyfluorescein Diacetate derivatives can be used as staining agents for flow cytometry to visualize cells undergoing apoptosis or necrosis. The expression is: 6-CARBOXYFLUORESCEIN DIACETATE is used as a staining agent for [visualizing cells undergoing apoptosis or necrosis] in the context of [flow cytometry] because [its derivatives can be used to specifically label and identify these cells].
Check Digit Verification of cas no
The CAS Registry Mumber 3348-03-6 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 3,3,4 and 8 respectively; the second part has 2 digits, 0 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 3348-03:
(6*3)+(5*3)+(4*4)+(3*8)+(2*0)+(1*3)=76
76 % 10 = 6
So 3348-03-6 is a valid CAS Registry Number.
InChI:InChI=1/C25H16O9/c1-12(26)31-15-4-7-18-21(10-15)33-22-11-16(32-13(2)27)5-8-19(22)25(18)20-9-14(23(28)29)3-6-17(20)24(30)34-25/h3-11H,1-2H3,(H,28,29)
3348-03-6Relevant articles and documents
Separating the isomers - Efficient synthesis of the N-hydroxysuccinimide esters of 5 and 6-carboxyfluorescein diacetate and 5 and 6-carboxyrhodamine B
Brunet, Aurlie,Aslam, Tashfeen,Bradley, Mark
, p. 3186 - 3188 (2014)
Diacetate protection of 5 and 6-carboxyfluorescein followed by synthesis of the N-hydroxysuccinimide esters allowed ready separation of the two isomers on a multi-gram scale. The 5 and 6-carboxyrhodamine B N-hydroxysuccinimide esters were also readily synthesised and separated.
Site-specific, reversible and fluorescent immobilization of proteins on CrAsH-modified surfaces for microarray analytics
Schulte-Zweckel, Janine,Rosi, Federica,Sreenu, Domalapally,Schr?der, Hendrik,Niemeyer, Christof M.,Triola, Gemma
supporting information, p. 12761 - 12764 (2015/05/20)
A novel technique for protein immobilization onto CrAsH-modified surfaces is presented. This approach enables an efficient, reversible and fluorogenic immobilization of proteins. Moreover, expressed proteins can also be directly immobilized from cellular lysates without prior purification. The immobilized proteins are suitable for protein-protein interaction studies and the fluorescence enhancement upon immobilization can be employed for the direct detection of the immobilized protein without the need for secondary detection methods. This journal is
