34336-93-1Relevant articles and documents
Preparation and Enzymatic Hydrolysis of Maltosyl-α-cyclodextrin
Sakano, Yoshiyuki,Sano, Mutsumi,Kobayashi, Tsuneo
, p. 3391 - 3398 (2007/10/02)
Maltosyl-α-cyclodextrin (6-α-maltosylcyclomaltohexaose, M-CD) was prepared from maltose and α-cyclodextrin by the reverse action of Bacillus pullulanase, and the action of α-amylases on this dextrin was examined.Among α-amylases tested, Thermoactinomyces vulgaris α-amylase (TVA) and Taka-amylase A (TAA) were found to attack the M-CD.Their action pattern on M-CD was studied.These α-amylases cleaved, first the cyclodextrin ring of M-CD, and the branched octasaccharides formed were immediately degraded to form glucose, branched tetraose, or pentaose, though the action pattern was different for TVA and TAA.In addition, TAA also split M-CD into glucose and glucosyl-α-cyclodextrin.Fission products at various stages of the reaction were separated and analyzed by paper chromatography and high performance liquid chromatography, their structures were analyzed, and the degradation pattern of M-CD was found.