3483-12-3 Usage
Description
DL-1,4-Dithiothreitol (DTT) is a threo isomer of 2,3-dihydroxy-1,4-dithiolbutane and an isomer of 1,4-dithioerythritol. It is a small-molecule redox reagent, also known as Cleland's reagent, which is used in molecular biology to maintain sulfhydryl (-SH) groups in the reduced state and for quantitative reduction of disulfide (-S-S-) groups. DTT is a white crystalline powder that is highly soluble in water and has a reduced odor compared to previous thiol compounds.
Uses
Used in Molecular Biology:
DL-1,4-Dithiothreitol is used as a reducing agent for maintaining sulfhydryl groups in the reduced state and for quantitative reduction of disulfide groups in molecular biology applications.
Used in Clinical Microbiology:
DL-1,4-Dithiothreitol is used as a protein denaturant to liquefy respiratory specimens and detach biofilm from orthopaedic prosthesis, aiding in the prompt diagnosis of indwelling device-related infections.
Used in Biochemistry and Biotechnology:
DL-1,4-Dithiothreitol is used as a water-soluble reducing reagent for various applications, such as reducing disulfides to generate sulfhydryls, reduction of proteins before SDS-PAGE analysis, studies of protein structure and function, keeping sulfhydryl groups of biomolecules in the reduced state to protect enzymes or receptors, living cells under ionizing radiations, and preventing the fading of fluorescence in labeled conjugates.
Used in Pharmaceutical Industry:
DL-1,4-Dithiothreitol is used as a reagent reducing disulfide bonds and as a protein denaturant, which can be beneficial in the development and production of pharmaceuticals that require these properties.
Biochem/physiol Actions
DL-Dithiothreitol denatures proteins by reducing the disulphide bonds and reducing them to SH groups. It also prevents intramolecular and intermolecular disulfide bond formation between cysteine residues of proteins.
storage
Store lyophilized at 4°C, desiccated. In lyophilized form, the chemical is stable for 12 months. Once in solution, store at -20°C and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.DTT solutions should be prepared fresh daily. If improperly stored (including room temperature and solution forms) its reducing ability may be reduced. Exposure to air should be minimized, even though DTT has a lower tendency to be oxidized directly by air than other reducing agents.Recorded half-life of DTT solutions at various pH and temperatures (all are in M potassium phosphate buffer):pH 6.5 @ 20°C= 40 hourspH 7.5 @ 20°C = 10 hourspH 8.5 @ 20°C = 1.4 hourspH 8.5 @ 0°C = 11 hourspH 8.5 @ 40°C = 0.2 hourspH 8.5 @20°C (+0.1 mM Cu2+) = 0.6 hourspH 8.5 @20°C (+0.1 mM EDTA) = 4 hours
Check Digit Verification of cas no
The CAS Registry Mumber 3483-12-3 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 3,4,8 and 3 respectively; the second part has 2 digits, 1 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 3483-12:
(6*3)+(5*4)+(4*8)+(3*3)+(2*1)+(1*2)=83
83 % 10 = 3
So 3483-12-3 is a valid CAS Registry Number.
InChI:InChI=1/C4H10O2S2/c1-2(5)3(6)4(7)8/h2-8H,1H3
3483-12-3Relevant articles and documents
Charge Accumulation and Multi-Electron Photoredox Chemistry with a Sensitizer–Catalyst–Sensitizer Triad
Nomrowski, Julia,Guo, Xingwei,Wenger, Oliver S.
, p. 14084 - 14087 (2018/09/11)
Photoinduced electron transfer in donor–sensitizer–acceptor compounds usually leads to simple electron–hole pairs, and photoredox catalysis typically relies on single-electron transfer (SET) events. This work reports on a molecular triad able to accumulate two electrons on a central dibenzo[1,2]dithiin moiety flanked by two peripheral RuII photosensitizers. Under continuous illumination, the doubly reduced form of the dibenzo[1,2]dithiin undergoes thiolate–disulfide exchange with an aliphatic disulfide substrate, thereby acting as a two-electron catalyst after two initial SET events with triethylamine at the RuII sensitizers. The use of a relatively simple triad for coupling two separate SET processes to a subsequent two-electron reduction is an important conceptual advance from photoinduced SET and light-driven charge accumulation towards multi-electron photoredox catalysis. This is relevant for artificial photosynthesis and light-driven multi-electron chemistry in general.
4-hydroxyquinoline-3-carboxamides and hydrazides as antiviral agents
-
, (2008/06/13)
The present invention provides 4-hydroxyquinoline-3-carboxamide and hydrazide compounds of formula I These compounds are useful to treat or prevent the herpesviral infections, particularly, human cytomegaloviral infection.
BIOLOGICALLY ACTIVE COMPOUNDS ISOLATED FROM AEROBIC FERMENTATION OF TRICHODERMA VIRIDE
-
, (2008/06/13)
This invention relates to compounds of structural formula (I) isolated from an aerobic fermentation of Trichoderma viride MF5628, ATCC 74084: (I) which are squalene synthase inhibitors and thus useful as cholesterol lowering agents. These compounds are also potent antifungal agents. Additionally, they inhibit farnesyl protein transferase and farnesylation of the oncogene protein Ras and are thus useful in treating cancer. This invention also relates to a process for obtaining compounds of structural formula (I)
