35061-50-8 Usage
Description
N,N'-Diacetylchitobiose, also known as Diacetylchitobiose, is a dimer of β(1,4) linked N-acetyl-D glucosamine. It is a white solid with various applications in different industries due to its unique chemical properties and interactions with other molecules.
Uses
Used in Pharmaceutical Industry:
N,N'-Diacetylchitobiose is used as an inhibitor of lysozyme, which is an enzyme that breaks down the cell walls of bacteria. This application is particularly useful in the treatment of Escherichia coli sepsis in dogs, as it helps reverse myocardial depression and lessens norepinephrine requirements.
Used in Immunology Research:
As a lectin inhibitor and immunopotentiator, N,N'-Diacetylchitobiose plays a crucial role in the study of the immune system. It helps researchers understand the interactions between lectins and other molecules, which can lead to the development of new therapies for various immune-related conditions.
Used in Microbiology and Fermentation Research:
N,N'-Diacetylchitobiose is used as an alternative source of N-acetylglucosamine by some bacteria. It is particularly useful in fermentation research, where it helps scientists study, differentiate, and characterize chitobiose transporter systems and enzymes such as β-N-acetylglucosaminidase(s) and chitobiose phosphorylase(s). This knowledge can be applied to improve the efficiency of fermentation processes and the production of various biotechnological products.
Biochem/physiol Actions
In chitinolytic bacteria, such as Vibrio, Streptomyces and Serratia, N,N′-Diacetylchitobiose (GlcNAc2) is not only a major breakdown product of chitinase, but it is also the smallest substance that induces chitinase production. It can also be utilized as a carbon source by E. coli, which does not express chitinases, but is exposed to GlcNAc2 produced by intestinal chitinolytic bacteria. In most organisms, the uptake of GlcNAc2 occurs via the phosphoenolpyruvate:glycose phosphotransferase system (PTS). GlcNAc2 has also been used as a substrate or inhibitor to study the activity of glycolytic enzymes.
Purification Methods
Recrystallise N,N'-Diacetylchitobiose from aqueous MeOH or aqueous EtOH/1,2-dimethoxyethane. [Zilliken et al. J Chem Soc 77 1296 1955, Beilstein 18/11 V 147.]
Check Digit Verification of cas no
The CAS Registry Mumber 35061-50-8 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 3,5,0,6 and 1 respectively; the second part has 2 digits, 5 and 0 respectively.
Calculate Digit Verification of CAS Registry Number 35061-50:
(7*3)+(6*5)+(5*0)+(4*6)+(3*1)+(2*5)+(1*0)=88
88 % 10 = 8
So 35061-50-8 is a valid CAS Registry Number.
35061-50-8Relevant articles and documents
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Zilliken et al.
, p. 1296 (1955)
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Cloning and expression of chitinase a from serratia marcescens for large-scale preparation of N,N-Diacetyl chitobiose
Wu, Yue-Jin,Cheng, Chih-Yu,Li, Yaw-Kuen
experimental part, p. 688 - 695 (2010/08/07)
The gene of Serratia marcescens chitinase A(chiA) was cloned by PCR. The complete gene was constructed into a pRSET vector and expressed in Escherichia coli. The recombinant enzyme was purified to > 90% homogeneity by hydrophobic interaction chromatograph
