3513-03-9 Usage
Description
Blasticidin S Hydrochloride, also known as BLASTICIDIN S HCl, is a peptidyl nucleoside antibiotic that specifically inhibits protein synthesis in both prokaryotes and eukaryotes. It is a white powder derived from the amphoteric nucleoside blasticidin S, which is produced by several species of Streptomyces. BLASTICIDIN S HYDROCHLORIDE was first reported in the late 1950s and has been used for various applications due to its ability to inhibit protein synthesis and induce apoptosis.
Uses
Used in Antifungal Applications:
BLASTICIDIN S HYDROCHLORIDE is used as an antifungal agent for the control of the rice pathogen, Piricularia oryzae. It exhibits potent activity against this pathogen and was commercially used in Japan for some time.
Used in Antibacterial Applications:
BLASTICIDIN S HYDROCHLORIDE is used as an antibacterial agent, as it is active against various bacteria. It inhibits protein synthesis in bacteria, making it a useful tool in studying and controlling bacterial growth.
Used in Antitumor Applications:
BLASTICIDIN S HYDROCHLORIDE is used as an antineoplastic agent for studying protein synthesis at the level of peptide bond formation. It induces apoptosis in tumor cell lines, making it a valuable compound in cancer research and potential therapeutic applications.
Used in Nematode Control:
BLASTICIDIN S HYDROCHLORIDE is used as a control agent against nematodes due to its ability to inhibit protein synthesis and induce apoptosis in these organisms.
Used in Genetic Research and Strain Manipulation:
BLASTICIDIN S HYDROCHLORIDE is used as a selection agent for cells carrying plasmids that confer resistance to the antibiotic. The resistance is conferred by the blasticidin S deaminase gene (bsr), which converts BLASTICIDIN S to a non-toxic deaminohydroxy derivative. This property makes it a valuable tool in genetic research and strain manipulations.
Used in Drug Transport and Uptake Studies:
BLASTICIDIN S HYDROCHLORIDE is used in studying the ABC-type transporter NppA1A2BCD, which is required for the uptake of BLASTICIDIN S and other peptidyl nucleoside antibiotics in Pseudomonas aeruginosa PA14. This application helps in understanding the mechanisms of drug transport and uptake in bacteria.
References
1) Svidritskiy?et al., (2013),?Blasticidin S inhibits translation by trapping deformed tRNA on the ribosome;?Proc. Natl. Acad. Sci. USA?110?12283
2) Pletzer?et al.?(2015),?The Pseudomonas aeruginosa PA14 ABC Transporter NppA1A2BCD is Required for Uptake of Peptidyl Nucleoside Antibiotics; J. Bacteriol.?197?2217
3) Asada?et al.?(2015),?Transfection of Babesia bovis by Double Selection with WR99210 and Blasticidin-S and Its Application for Functional Analysis of Thioredoxin Peroxidase-1; PLoS One,?10?e0125993
4) Garg and Qadri (2010),?Hemoglobin transforms anti-inflammatory Salmonella typhi virulence polysaccharide into a TLR-2 agonist; J. Immunol.?184?5980
Check Digit Verification of cas no
The CAS Registry Mumber 3513-03-9 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 3,5,1 and 3 respectively; the second part has 2 digits, 0 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 3513-03:
(6*3)+(5*5)+(4*1)+(3*3)+(2*0)+(1*3)=59
59 % 10 = 9
So 3513-03-9 is a valid CAS Registry Number.
InChI:InChI=1/C17H26N8O5.ClH/c1-24(16(20)21)6-4-9(18)8-12(26)22-10-2-3-13(30-14(10)15(27)28)25-7-5-11(19)23-17(25)29;/h2-3,5,7,9-10,13-14H,4,6,8,18H2,1H3,(H3,20,21)(H,22,26)(H,27,28)(H2,19,23,29);1H/t9-,10+,13-,14+;/m1./s1
