371942-69-7 Usage
Uses
Used in Pharmaceutical Industry:
6-Amino-N-[3-[4-(4-morpholinyl)pyrido[3',2':4,5]furo[3,2-d]pyrimidin-2-yl]phenyl]-3-pyridinecarboxamide is used as a selective inhibitor for [phosphatidylinositol phosphate kinase PIP5KIII (PIKfyve)] for [its ability to significantly reduce the survival of primary mouse hippocampal neurons in culture and increase the level of the autophagosomal marker protein LC3-II].
Used in Neurodegenerative Research:
In the field of neurodegenerative research, 6-Amino-N-[3-[4-(4-morpholinyl)pyrido[3',2':4,5]furo[3,2-d]pyrimidin-2-yl]phenyl]-3-pyridinecarboxamide is used as a research tool for [studying the role of autophagy and neuronal survival] due to its effect on primary mouse hippocampal neurons and the autophagosomal marker protein LC3-II.
Used in Cellular Transport Studies:
6-Amino-N-[3-[4-(4-morpholinyl)pyrido[3',2':4,5]furo[3,2-d]pyrimidin-2-yl]phenyl]-3-pyridinecarboxamide is used as a disruptor of [cellular endosomal transport] for [studying the effects of PtdIns(3,5)P2 inhibition on cellular processes] as it has been shown to block serum-induced PtdIns(3,5)P2 production in NIH3T3 cells.
Used in Drug Development:
In the drug development industry, 6-Amino-N-[3-[4-(4-morpholinyl)pyrido[3',2':4,5]furo[3,2-d]pyrimidin-2-yl]phenyl]-3-pyridinecarboxamide is used as a lead compound for [developing new therapeutics targeting PtdInsP kinase] because of its high selectivity and potency against mammalian type III PtdInsP kinase.
Biological Activity
ym201636 is a potent, selective and cell-permeable inhibitor of mammalian class iii phosphatidylinositol phosphate kinase pikfyve with ic50 value of 33nm [1].the pyridofuropyrimidine compound, ym201636, is a small-molecule inhibitor of pikfyve. in the in vitro assay, it inhibits pikfyve with ic50 value of 33nm and shows no inhibition of yeast orthologue of pikfyve. it inhibits ptdins3p p110α and type iα ptdinsp kinase with ic50 values of 3μm and > 2μm, respectively. the type iiγ ptdinsp kinase is found to be insensitive to ym201636. in serum-starved nih3t3 cells, ym201636 inhibits ptdins (3, 5) p2 production by 80% at concentration of 800nm. acute treatment of ym201636 to mefs, mdck, mcf10a, cos7 and nih3t3 cells causes the formation of large vesicular structures. besides that, ym201636 is also found to reduce the akt phosphorylation and glut4 cell surface translocation through inhibiting the insulin-dependent class i pi 3k activation [1, 2].
references
[1] jefferies h b j, cooke f t, jat p, et al. a selective pikfyve inhibitor blocks ptdins (3, 5) p2 production and disrupts endomembrane transport and retroviral budding. embo reports, 2008, 9(2): 164-170.[2] ikonomov o c, sbrissa d, shisheva a. ym201636, an inhibitor of retroviral budding and pikfyve-catalyzed ptdins (3, 5) p2synthesis, halts glucose entry by insulin in adipocytes. biochemical and biophysical research communications, 2009, 382(3): 566-570.
Check Digit Verification of cas no
The CAS Registry Mumber 371942-69-7 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 3,7,1,9,4 and 2 respectively; the second part has 2 digits, 6 and 9 respectively.
Calculate Digit Verification of CAS Registry Number 371942-69:
(8*3)+(7*7)+(6*1)+(5*9)+(4*4)+(3*2)+(2*6)+(1*9)=167
167 % 10 = 7
So 371942-69-7 is a valid CAS Registry Number.
