39382-08-6 Usage
Description
Phosphatidylethanolamine, also known as L-α-Phosphatidylethanolamine, is a type of phospholipid that is commonly found in egg yolk. It plays a crucial role in the structure and function of cell membranes and has various applications in different fields.
Uses
Used in the Synthesis of Giant Unilamellar Vesicles (GUVs):
Phosphatidylethanolamine is used as a component of lipid mixture for the synthesis of giant unilamellar vesicles (GUVs). GUVs are large, single-compartment vesicles that are used to study the properties of lipid bilayers and membrane proteins.
Used in Ceramide Phosphoethanolamine (CPE) Synthase Assay:
Phosphatidylethanolamine is used in the ceramide phosphoethanolamine (CPE) synthase assay, which involves the use of crude sphingomyelin synthase-related protein from Hela cells. This assay is important for studying the biosynthesis of ceramide phosphoethanolamine, a bioactive lipid involved in various cellular processes.
Used in Lipid Quantification:
Phosphatidylethanolamine is used for the standard curve generation for the quantification of lipids in plantaris muscle homogenates from mice. This application is important for understanding the lipid composition and metabolism in muscle tissues, which can have implications for various diseases and conditions related to lipid metabolism.
Enzyme inhibitor
This hygroscopic phospholipid, also called cephalin and 1,2-diacyl-snglycero- 3-phosphoethanolamine, is an important constituent of biomembranes and lipoproteins. It is the major structural phospholipid in brain tissue. The physical properties depend on the nature of the acyl groups and on the presence of other lipids. Freshly prepared phosphatidylethanolamines are white solids that turn yellow and brown on exposure to air. They typically sinter between 80 and 90°C and melt in the neighborhood of 175°C. Solutions of phosphatidylethanolamine slowly decompose at room temperature (roughly 0.3-0.5% per day); hence, solutions should always be freshly prepared and kept cold prior to use. It is labile in alkaline conditions. See also specific compound Target(s): b- N-acetylglucosaminyl-glycopeptide b-1,4-galactosyltransferase; Nacetyllactosamine synthase; acylglycerol kinase, or monoacylglycerol kinase; cholesterol monooxygenase, side-chain-cleaving, or CYP11A1; chymase; cytidylate cyclase; dolichyldiphosphatase; dolichyl-phosphatase; dopamine b-monooxygenase; ethanolaminephosphotransferase; glutamate dehydrogenase; γ-glutamyl transpeptidase, mildly inhibited; glycerol-3-phosphate Oacyltransferase; glycerone-phosphate O-acyltransferase, or dihydroxyacetione-phosphate O-acyltransferase; hormone-sensitive lipase; 3-hydroxybutyrate dehydrogenase; indole-3-acetate b-glucosyltransferase; phosphatidate cytidylyltransferase; 1- phosphatidylinositol 4-kinase; phosphoinositide phospholipase C; phospholipase D, phosphatidylcholine-specific enzyme; sphingomyelin phosphodiesterase; steryl-b-glucosidase; UDP-Nacetylglucosamine: dolichyl-phosphate N-acetylglucosaminephosphotransferase.
Purification Methods
Purify the cephalin by dissolving it in EtOH, adding Pb(OAc)2.3H3O (30g in 100mL H2O) until excess Pb2+ is present. Filter off the solid. Pass CO2 gas through the solution until precipitation of PbCO3 ceases. Filter the solid off and evaporate (while bubbling CO2) under vacuum. An equal volume of H2O is added to the residual oil and extracted with hexane. The hexane extract is washed with H2O until the aqueous phase is free from Pb [test with dithizone (2 mg in 100 mL CCl4; Feigel Spot Tests Vol I, Elsevier p. 10 1954)]. The hexane is dried (Na2SO4), filtered and evaporated to give a yellow waxy solid which should be dried to constant weight in vacuo. It is practically insoluble in H2O and Me2CO, but freely soluble in CHCl3 (5%) and Et2O, and slightly soluble in EtOH. [Schofield & Dutton Biochemical Preparations 5 5 1957.]
Check Digit Verification of cas no
The CAS Registry Mumber 39382-08-6 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 3,9,3,8 and 2 respectively; the second part has 2 digits, 0 and 8 respectively.
Calculate Digit Verification of CAS Registry Number 39382-08:
(7*3)+(6*9)+(5*3)+(4*8)+(3*2)+(2*0)+(1*8)=136
136 % 10 = 6
So 39382-08-6 is a valid CAS Registry Number.