41085-99-8

Basic information

• Product Name: 1,1'-DIOCTADECYL-3,3,3',3'-TETRAMETHYLINDOCARBOCYANINE PERCHLORATE
• Synonyms: DII (DYE);DII;DIL;DILC18(3);D 282;D 282 (DYE);3H-INDOLIUM, 2-[3-(1,3-DIHYDRO-3,3-DIMETHYL-1-OCTADECYL-2H-INDOL-2-YLIDENE)-1-PROPENYL]-3,3-DIMETHYL-1-OCTADECYL-, PERCHLORATE;2-[(E)-3-(3,3-DIMETHYL-1-OCTADECYL-1,3-DIHYDRO-2H-INDOL-2-YLIDENE)-1-PROPENYL]-3,3-DIMETHYL-1-OCTADECYL-3H-INDOLIUM PERCHLORATE
• CAS NO: 41085-99-8
• Molecular Formula: C59H97ClN2O4
• Molecular Weight: 933.87
• Product Categories: Cyanine
• Mol File: 41085-99-8.mol
• Article Data: 0

Chemical Properties

• Melting Point: 68 °C (dec.)(lit.)
• Appearance: Burgundy to purples-violet/crystals or powder
• Storage Temp.: 2-8°C
• Solubility: DMF: soluble
• BRN: 8251870
• CAS DataBase Reference: 1,1'-DIOCTADECYL-3,3,3',3'-TETRAMETHYLINDOCARBOCYANINE PERCHLORATE(CAS DataBase Reference)
• NIST Chemistry Reference: 1,1'-DIOCTADECYL-3,3,3',3'-TETRAMETHYLINDOCARBOCYANINE PERCHLORATE(41085-99-8)
• EPA Substance Registry System: 1,1'-DIOCTADECYL-3,3,3',3'-TETRAMETHYLINDOCARBOCYANINE PERCHLORATE(41085-99-8)

Safety Data

• Safety Statements: 22-24/25
• WGK Germany: 3
• F: 8
• Hazardous Substances Data: 41085-99-8(Hazardous Substances Data)

Usage

Description

1,1'-Dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate, commonly known as DiI, is a fluorescent lipophilic cationic indocarbocyanine dye typically used as a perchlorate salt. It is characterized by its ability to stain and label various biological structures, making it a valuable tool in scientific research and imaging.

Uses

Used in Neurobiology:
1,1'-Dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate is used as a retrograde stain for neurons, providing intense, long-lasting staining of live neurons both in vivo and in vitro. It is relatively nontoxic to cell bodies and is retained in neurons, making it an ideal tool for studying neuronal structures and functions.
Used in Membrane Studies:
DiI is employed as a lipophilic dye to record membrane voltage and for studies of membrane fluidity. It is particularly useful in staining neurons in vitro and in vivo, as it allows for the visualization and analysis of membrane properties.
Used in Cell Labeling:
1,1'-Dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate has been used in the fluorescent labeling of mesenchymal stem cells (MSCs) and the cerebral cortex. This application enables researchers to track and study the behavior of these cells in various biological processes.
Used in Electromyogram (EMG) Studies:
DiI is utilized in electromyogram (EMG) research to determine awake and anesthetized states in mice. This application aids in understanding the differences in muscle activity and electrical signals between these two states.

Biochem/physiol Actions

Lipophilic carbocyanine dye used primarily for optical recordings of membrane voltage and for studies of membrane fluidity. Retrograde stain for neurons; provides intense, long-lasting staining of live neurons in vivo and in vitro.