432552-01-7Relevant articles and documents
Synthesis of all-trans-[10′-3H]-8′-apo-β-carotenoic acid
Reddy, Pulgam Veera,Rabago-Smith, Monsterrat,Borhan, Babak
, p. 79 - 89 (2002)
The enzyme, 15, 15′-β-carotene dioxygenase (BCDOX), facilitates the oxidation of β-carotene to yield retinal. This is a remarkable process in which one of 11 double bonds in β-carotene is selectively oxidized. To further probe the mechanistic aspects of BCDOX, the synthesis of all-trans-[10′-3H]-8′-apo-β-carotenoic acid is reported. This compound will be used as a photoaffinity labeling reagent to probe the β-carotene binding pocket within BCDOX. The synthesis outlines a simple and efficient route for the incorporation of tritium at the 10′ olefinic carbon of 8′-apo-β-carotenoic acid. Copyright
