49863-47-0

Basic information

• Product Name: G418
• Synonyms: O-2-AMINO-2,7-DIDEOXY-DELTA-GLY-CERO-ALPHA-D-GLUCO-HEPTO-PYRANOSYL[1->4]-O-3-DEOXY-4C-METHYL-3-[METHYL-AMINO]-BETA-L-ARABINOPYRONOSYL-DELTA-STREPTAMINE, DISULFATE SALT;AMINOGLYCOSIDE ANTIBIOTIC;ANTIBIOTIC G418;ANTIBIOTIC G418 DISULFATE;ANTIBIOTIC G418 DISULFATE SALT;GENETICIN DISULFATE;GENETICIN DISULFATE SALT;GENETICIN(R)
• CAS NO: 49863-47-0
• Molecular Formula: C₂₀H₄₀N₄O₁₀
• Molecular Weight: 496.55
• Mol File: 49863-47-0.mol
• Article Data: 0

Chemical Properties

• Boiling Point: 760.3 °C at 760 mmHg
• Flash Point: 413.6 °C
• Appearance: /white powder
• Density: 1.47 g/cm³
• Vapor Pressure: 1.1E-26mmHg at 25°C
• Refractive Index: 1.625
• Storage Temp.: 2-8°C
• Solubility: H2O: 50 mg/mL
• PKA: 13.29±0.70(Predicted)
• Stability: Hygroscopic
• CAS DataBase Reference: G418(CAS DataBase Reference)
• NIST Chemistry Reference: G418(49863-47-0)
• EPA Substance Registry System: G418(49863-47-0)

Safety Data

• Hazard Codes: Xn
• Statements: 20/21/22
• Safety Statements: 22-24/25
• WGK Germany: 3
• Hazardous Substances Data: 49863-47-0(Hazardous Substances Data)

Usage

Description

G418, also known as Geneticin Disulfate Salt, is an aminoglycoside antibiotic that is structurally similar to Gentamicin B1. It is produced by the bacterium Micromonospora rhodorangea and is known for its ability to block polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells.

Uses

Used in Genetic Engineering:
G418 is used as a selective agent for the maintenance of neomycin phosphotransferase (NPT II) gene in genetically modified organisms. It is particularly useful in the selection of successfully transformed cells, as it inhibits the growth of non-resistant cells, allowing for the identification and isolation of those with the desired genetic modifications.
Used in Cancer Research:
In the field of cancer research, G418 is employed as a tool to study the mechanisms of drug resistance in cancer cells. By exposing cancer cells to G418, researchers can investigate how these cells develop resistance to the antibiotic and potentially identify new targets for cancer therapies.
Used in Cell Culture:
G418 is used as a supplement in cell culture media to select for cells that have been genetically modified to express the NPT II gene. This allows researchers to maintain a population of cells with the desired genetic traits, facilitating various types of cell culture experiments and studies.
Used in Pharmaceutical Industry:
In the pharmaceutical industry, G418 is utilized as a component in the development of new drugs and therapies. Its ability to inhibit protein synthesis makes it a valuable tool for studying the effects of various compounds on cellular processes and for identifying potential drug candidates with novel mechanisms of action.

InChI:InChI=1/C20H40N4O10/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19/h6-19,24-30H,4-5,21-23H2,1-3H3/t6-,7+,8-,9-,10-,11+,12+,13-,14-,15-,16+,17-,18-,19-,20+/m1/s1