518-67-2

Basic information

• Product Name: Dimidium bromide
• Synonyms: DIMIDIUM BROMIDE;DIMIDIUM BROMIDE 1/2 H2O;2,7-DIAMINO-10-METHYL-9-PHENYLPHENANTHRIDINIUM BROMIDE;3,5-DIAMINO-5-METHYL-6-PHENYLPHENANTHRIDINIUM BROMIDE;3,8-DIAMINO-5-METHYL-6-PHENYLPHENANTHRIDINIUM BROMIDE;3,8-DIAMINO-5-METHYL-6-PHENYLPHENANTHRIDIUM BROMIDE;TRYPADINE;3,8-diamino-5-methyl-6-phenyl-phenanthridiniubromide
• CAS NO: 518-67-2
• Molecular Formula: Br*C₂₀H₁₈N₃
• Molecular Weight: 380.28
• EINECS: 208-256-7
• Product Categories: Heterocyclic Compounds;Fluorescent Enzyme Substrates;Fluorescent Enzyme Substrates for Electrophoresis;Fluorescent Stains;Nucleic Acid Stains;Alphabetical;Biochemicals and Reagents;Building Blocks;Chemical Synthesis;Fluorescent Probes;Heterocyclic Building Blocks;Labels;N-Containing;Others;Particles and Stains;Amines;Aromatics;Inhibitors;Intermediates & Fine Chemicals;Pharmaceuticals;Marker
• Mol File: 518-67-2.mol
• Article Data: 0

Chemical Properties

• Melting Point: 243-248 °C(lit.)
• Boiling Point: °Cat760mmHg
• Flash Point: °C
• Appearance: Dark red to purple/Powder
• Density: g/cm³
• Storage Temp.: 2-8°C
• Solubility: Methanol (Slightly), Water (Slightly)
• Water Solubility: Soluble in water.
• BRN: 3809779
• CAS DataBase Reference: Dimidium bromide(CAS DataBase Reference)
• NIST Chemistry Reference: Dimidium bromide(518-67-2)
• EPA Substance Registry System: Dimidium bromide(518-67-2)

Safety Data

• Hazard Codes: Xi,Xn
• Statements: 36/37/38-40
• Safety Statements: 26-36-22
• RIDADR: 2811
• WGK Germany: 3
• RTECS: SF7960500
• F: 3-8-10
• HazardClass: 6.1(b)
• PackingGroup: III
• Hazardous Substances Data: 518-67-2(Hazardous Substances Data)

Usage

Description

Dimidium bromide, also known as Disulphine Blue, is a dark red crystalline compound with a slight solubility in water and solubility in organic solvents such as chloroform, dichloromethane, and methanol. It is widely recognized for its applications in various scientific and industrial fields due to its unique properties.

Uses

Used in Cell Growth Inhibition:
Dimidium bromide is used as an inhibitor of L-cell growth, making it a valuable tool in cellular research and development.
Used as a Fluorescent Probe for Nucleic Acids:
Dimidium bromide serves as a fluorescent probe for nucleic acids, which is essential in molecular biology and genetic research.
Used as an Interchelating Agent:
It is also utilized as an interchelating agent, playing a crucial role in various chemical reactions and processes.
Used in the Determination of Cationic Surfactants:
Dimidium bromide is employed in the determination of cationic surfactants, which is vital in the field of chemistry and material science.
Used as a General Chemical Reagent:
Dimidium Bromide Disulphine Blue Indicator is used as a general chemical reagent, highlighting its versatility and wide range of applications.
Used in Anionic Surfactants Determination:
It is specifically used in the two-phase method for anionic surfactants determination, further expanding its utility in the chemical industry.
Application Industry:
The applications of Dimidium bromide span across various industries, including pharmaceuticals, biotechnology, molecular biology, genetic research, and material science, among others. Each of these industries benefits from the unique properties and functions of Dimidium bromide, making it an indispensable compound in contemporary scientific research and development.

InChI:InChI=1/C20H17N3.BrH/c1-23-19-12-15(22)8-10-17(19)16-9-7-14(21)11-18(16)20(23)13-5-3-2-4-6-13;/h2-12,22H,21H2,1H3;1H

Synthetic route

6-butoxy-5-methyl-6-phenyl-5,6-dihydro-phenanthridine-3,8-diyldiamine (875227-05-7) → 3,8-diamino-5-methyl-6-phenyl-phenanthridinium; bromide (518-67-2)

Conditions	Yield
With hydrogen bromide

2-amino-4-chloro-1,6-dimethyl-pyrimidinium; iodide + 3,8-diamino-5-methyl-6-phenyl-phenanthridinium; bromide (518-67-2) → 3-amino-8-(2-amino-1,6-dimethyl-pyrimidinium-4-ylamino)-5-methyl-6-phenyl-phenanthridinium; diiodide

Conditions	Yield
anschliessend mit KI;

2-amino-4-chloro-1,6-dimethyl-pyrimidinium; iodide + 3,8-diamino-5-methyl-6-phenyl-phenanthridinium; bromide (518-67-2) → 3,8-bis-(2-amino-1,6-dimethyl-pyrimidinium-4-ylamino)-6-(4-chloro-phenyl)-5-methyl-phenanthridinium; tris iodide

Conditions	Yield
anschliessend mit KI;

Upstream product

• 875227-05-7 6-butoxy-5-methyl-6-phenyl-5,6-dihydro-phenanthridine-3,8-diyldiamine