51811-82-6

Basic information

• Product Name: Giemsa stain
• Synonyms: Giemsa's stain: (& blood stain);Azure eosin methylene blue, Giemsa solution;Giemsa stain, modified;3-(Dimethylamino)-7-aminophenothiazin-5-ium chloride;Giemsa Stain,pure,high purity biological stain;Giemsa Stain,pure,certified;Azure mixture sicc;Giemsa stain,Azure mixture sicc. Giemsa stain
• CAS NO: 51811-82-6
• Molecular Formula: C14Cl1H14N3S1
• Molecular Weight: 291.8
• EINECS: 257-438-2
• Product Categories: UVCBs-organic
• Mol File: 51811-82-6.mol
• Article Data: 0

Chemical Properties

• Melting Point: 300 °C(lit.)
• Boiling Point: 442.8 °C at 760 mmHg
• Flash Point: 15 °C
• Appearance: Greenish-gold/powder
• Density: 0.99
• Vapor Pressure: 3.03E-08mmHg at 25°C
• Storage Temp.: Store at RT.
• Solubility: ethanol: 1 mg/mL
• Water Solubility: Insoluble in water (<0.1%), methanol (5.8 mg/L), and ethanol.
• CAS DataBase Reference: Giemsa stain(CAS DataBase Reference)
• NIST Chemistry Reference: Giemsa stain(51811-82-6)
• EPA Substance Registry System: Giemsa stain(51811-82-6)

Safety Data

• Hazard Codes: Xi,T,F,Xn
• Statements: 41-39/23/24/25-23/24/25-11-63-62-46-36/38-21-52/53-22
• Safety Statements: 7-16-36/37-45-39-26-53-25-61
• RIDADR: UN 1230 3/PG 2
• WGK Germany: 3
• F: 12
• TSCA: Yes
• Hazardous Substances Data: 51811-82-6(Hazardous Substances Data)

Usage

Description

Giemsa stain is a histological stain used for the examination of cells and tissues under a microscope. It is a modified version of the original Giemsa stain, with its pH adjusted from the usual 6.8 to 9.0. The stain is characterized by its dark green to greenish-black fine crystalline powder appearance.

Uses

1. Used in Medical and Biological Research:
Giemsa stain is used as a laboratory reagent and stain for microscopy. It is particularly useful for the staining of cells and blood films, allowing for the examination of various cellular structures and components.
2. Used in Histopathology:
Giemsa stain is employed in the histopathologic detection of malaria and other parasites such as spirochete and protozoan. Its ability to differentiate between infected and non-infected cells aids in the diagnosis of these diseases.
3. Used in Differential Staining:
The stain has been incorporated in differential staining between bacterial and human cells, helping to distinguish between the two types of cells and facilitating the study of bacterial infections.
4. Used in Combination with Other Stains:
Giemsa stain is often used in combination with May-Grunwald or Wright Stain (sc-203763) as a histological hematology stain. This combination enhances the visualization of blood cells and other hematological components, providing a more comprehensive analysis.

InChI:InChI=1/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H